Isolation and characterization of variable (GT)n repetitive sequences from Atlantic salmon, Salmo salar L.

Isolation and characterization of variable (GT)n repetitive sequences from Atlantic salmon, Salmo... Microsatellites are islands of long repeats of mono‐, di‐ or trinucleotides evenly distributed in the eukaryotic genome with an average distance of 50–100 kb. They display a high degree of length polymorphism and heterozygosity at individual loci, making them highly useful as markers in the development of genomic maps of eukaryotes. In the present work, we examined the dinucleotide repeat motif (dG‐dT)n in the Atlantic salmon, Salmo salar L., genome. The frequency of (dG‐dT)n microsatellites in salmon correlates well with earlier published estimations. Cloning and sequencing of 45 salmon microsatellites revealed perfect and imperfect repeats, but no compound microsatellites. The distribution of number of repeat units in salmon microsatellites differ significantly from that of higher vertebrates. Salmon tends to have more long repeat stretches and less intermediate length repeats. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Animal Genetics Wiley

Isolation and characterization of variable (GT)n repetitive sequences from Atlantic salmon, Salmo salar L.

Animal Genetics, Volume 24 (3) – Jun 1, 1993

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Publisher
Wiley
Copyright
Copyright © 1993 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0268-9146
eISSN
1365-2052
DOI
10.1111/j.1365-2052.1993.tb00287.x
Publisher site
See Article on Publisher Site

Abstract

Microsatellites are islands of long repeats of mono‐, di‐ or trinucleotides evenly distributed in the eukaryotic genome with an average distance of 50–100 kb. They display a high degree of length polymorphism and heterozygosity at individual loci, making them highly useful as markers in the development of genomic maps of eukaryotes. In the present work, we examined the dinucleotide repeat motif (dG‐dT)n in the Atlantic salmon, Salmo salar L., genome. The frequency of (dG‐dT)n microsatellites in salmon correlates well with earlier published estimations. Cloning and sequencing of 45 salmon microsatellites revealed perfect and imperfect repeats, but no compound microsatellites. The distribution of number of repeat units in salmon microsatellites differ significantly from that of higher vertebrates. Salmon tends to have more long repeat stretches and less intermediate length repeats.

Journal

Animal GeneticsWiley

Published: Jun 1, 1993

Keywords: ; ; ;

References

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