Interaction between the RP4 coupling protein TraG and the pBHR1 mobilization protein Mob

Interaction between the RP4 coupling protein TraG and the pBHR1 mobilization protein Mob It is currently believed that interaction between the relaxosome of a mobilizable plasmid and the transfer machinery of the helper conjugative plasmid is mediated by a TraG family coupling protein. The coupling proteins appear as an essential determinant of mobilization specificity and efficiency. Using a two‐hybrid system, we demonstrated for the first time the direct in vivo interaction between the coupling protein of a conjugative plasmid (the TraG protein of RP4) and the relaxase of a mobilizable plasmid (the Mob protein of pBHR1, a derivative of the broad host range plasmid pBBR1). This interaction was confirmed in vitro by an overlay assay and was shown to occur even in the absence of the transfer origin of pBHR1. We showed that, among 11 conjugative plasmids tested, pBHR1 is efficiently mobilized only by plasmids encoding an IncP‐type transfer system. We also showed that the RP4 TraG coupling protein is essential for mobilization of a pBBR1 derivative and is the element that allows its mobilization by R388 plasmid (IncW) at a detectable frequency. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Microbiology Wiley

Interaction between the RP4 coupling protein TraG and the pBHR1 mobilization protein Mob

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Publisher
Wiley
Copyright
Copyright © 2000 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0950-382X
eISSN
1365-2958
DOI
10.1046/j.1365-2958.2000.02077.x
Publisher site
See Article on Publisher Site

Abstract

It is currently believed that interaction between the relaxosome of a mobilizable plasmid and the transfer machinery of the helper conjugative plasmid is mediated by a TraG family coupling protein. The coupling proteins appear as an essential determinant of mobilization specificity and efficiency. Using a two‐hybrid system, we demonstrated for the first time the direct in vivo interaction between the coupling protein of a conjugative plasmid (the TraG protein of RP4) and the relaxase of a mobilizable plasmid (the Mob protein of pBHR1, a derivative of the broad host range plasmid pBBR1). This interaction was confirmed in vitro by an overlay assay and was shown to occur even in the absence of the transfer origin of pBHR1. We showed that, among 11 conjugative plasmids tested, pBHR1 is efficiently mobilized only by plasmids encoding an IncP‐type transfer system. We also showed that the RP4 TraG coupling protein is essential for mobilization of a pBBR1 derivative and is the element that allows its mobilization by R388 plasmid (IncW) at a detectable frequency.

Journal

Molecular MicrobiologyWiley

Published: Sep 1, 2000

References

  • Isolation and molecular characterization of a novel broad‐host‐range plasmid from Bordetella bronchiseptica with sequence similarities to plasmids from gram‐positive organisms
    Antoine, Antoine; Locht, Locht
  • Genetic evidence of a coupling role for the TraG protein family in bacterial conjugation
    Cabezón, Cabezón; Sastre, Sastre; De La Cruz, De La Cruz
  • Conjugal transmission of plasmids
    Clark, Clark; Warren, Warren
  • Biochemical characterization of Escherichia coli DNA helicase I
    Dash, Dash; Traxler, Traxler; Panicker, Panicker; Hackney, Hackney; Minkley, Minkley
  • Four new derivatives of the broad‐host‐range cloning vectors pBBR1MCS, carrying different antibiotic‐resistance cassettes
    Kovach, Kovach; Elzer, Elzer; Hill, Hill; Robertson, Robertson; Farris, Farris; Roop, Roop; Peterson, Peterson
  • DNA processing reactions in bacterial conjugation
    Lanka, Lanka; Wilkins, Wilkins
  • Construction of derivatives of the F plasmid pOX‐tra715: characterization of traY and traD mutants that can be complemented in trans
    Maneewannakul, Maneewannakul; Kathir, Kathir; Endley, Endley; Moore, Moore; Manchak, Manchak; Frost, Frost; Ippen‐Ihler, Ippen‐Ihler

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