Interaction between developmental and cell cycle regulators is required for morphogenesis in Aspergillus nidulans

Interaction between developmental and cell cycle regulators is required for morphogenesis in... In Aspergillus nidulans, mutation of the transcriptional regulator brlA arrests formation of asexual spore‐forming structures called conidiophores but does not hinder vegetative hyphal growth. During conidiophore development a 6‐fold, brlA‐dependent increase in the kinase activities of NIMXcdc2 and NIMA occurs. A similar level of kinase induction was promoted by ectopic expression of brlA. Northern and Western analysis revealed marked induction of nimXcdc2 mRNA after ectopic expression of brlA and increased amounts of NIMXcdc2. Therefore, nimXcdc2 is developmentally regulated by brlA indicating a direct role for brlA in the regulation of cell cycle genes. That correct regulation of nimXcdc2 is important for normal development was further supported by analysis of conidiophore development and septation in cell cycle specific mutants. Most noticeably, the nimXcdc2AF mutation promoted inappropriate septation and hindered the switch from filamentous growth to budding growth seen during conidiophore development. Therefore, in contrast to the situation previously reported for other multicellular eukaryotes, interaction between developmental regulators and cell cycle regulators is essential for normal morphogenesis in A.nidulans. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The EMBO Journal Wiley

Interaction between developmental and cell cycle regulators is required for morphogenesis in Aspergillus nidulans

Loading next page...
 
/lp/wiley/interaction-between-developmental-and-cell-cycle-regulators-is-2UNHDkBDqh
Publisher
Wiley
Copyright
Copyright © 2013 Wiley Periodicals, Inc
ISSN
0261-4189
eISSN
1460-2075
D.O.I.
10.1093/emboj/18.24.6994
Publisher site
See Article on Publisher Site

Abstract

In Aspergillus nidulans, mutation of the transcriptional regulator brlA arrests formation of asexual spore‐forming structures called conidiophores but does not hinder vegetative hyphal growth. During conidiophore development a 6‐fold, brlA‐dependent increase in the kinase activities of NIMXcdc2 and NIMA occurs. A similar level of kinase induction was promoted by ectopic expression of brlA. Northern and Western analysis revealed marked induction of nimXcdc2 mRNA after ectopic expression of brlA and increased amounts of NIMXcdc2. Therefore, nimXcdc2 is developmentally regulated by brlA indicating a direct role for brlA in the regulation of cell cycle genes. That correct regulation of nimXcdc2 is important for normal development was further supported by analysis of conidiophore development and septation in cell cycle specific mutants. Most noticeably, the nimXcdc2AF mutation promoted inappropriate septation and hindered the switch from filamentous growth to budding growth seen during conidiophore development. Therefore, in contrast to the situation previously reported for other multicellular eukaryotes, interaction between developmental regulators and cell cycle regulators is essential for normal morphogenesis in A.nidulans.

Journal

The EMBO JournalWiley

Published: Dec 15, 1999

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off