Inhibitory effect of a reproductive‐related serpin on sperm trypsin‐like activity implicates its role in sperm maturation of Penaeus monodon

Inhibitory effect of a reproductive‐related serpin on sperm trypsin‐like activity implicates... AbbreviationsBoc‐QAR‐MCAt‐butyloxycarbonyl‐QAR‐4‐methylcoumarin‐7‐amiderrPmserpinP. monodon reproductive tract‐related serine protease inhibitorrrPmSERPIN‐6Hisrecombinant rrPmSERPIN with carboxy‐terminal hexahistidinesserpinserine‐type protease inhibitorSuc‐AAPF‐MCAsuccinyl‐AAPF‐4‐methylcoumarin‐7‐amide.INTRODUCTIONThe black tiger shrimp Penaeus monodon is an economically important species of shrimp in Thailand that highly contributes to the value of seafood exports each year. The constant expansion of global shrimp demand has led to increases in stocking farms and a need for quality nauplii. Production of nauplii in hatcheries hinges on the high reproductive performance of broodstocks and high rates of fertilization. While artificial insemination in this species has been used to facilitate fertilization and breeding, the success of the technique is still dependent on sperm quality. In contrast to mammalian sperm, sperm quality in P. monodon has been difficult to assess due to their non‐motile nature and the lack of reliable fertility markers.Testicular spermatozoa generally lack fertilizing ability because they require activation by maturation factors that are secreted into the spermatic tract as well as in the female thelycum. For example, interactions of mammalian sperm with maturation or decapacitation factors in seminal vesicle fluid prevent premature capacitation and acrosome reaction; these are reversed in the oviduct, where sperm are activated by capacitation and undergo numerous additional changes (Fraser, ; Ni et al., ; Nixon http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Reproduction & Development Wiley

Inhibitory effect of a reproductive‐related serpin on sperm trypsin‐like activity implicates its role in sperm maturation of Penaeus monodon

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
© 2018 Wiley Periodicals, Inc.
ISSN
1040-452X
eISSN
1098-2795
D.O.I.
10.1002/mrd.22954
Publisher site
See Article on Publisher Site

Abstract

AbbreviationsBoc‐QAR‐MCAt‐butyloxycarbonyl‐QAR‐4‐methylcoumarin‐7‐amiderrPmserpinP. monodon reproductive tract‐related serine protease inhibitorrrPmSERPIN‐6Hisrecombinant rrPmSERPIN with carboxy‐terminal hexahistidinesserpinserine‐type protease inhibitorSuc‐AAPF‐MCAsuccinyl‐AAPF‐4‐methylcoumarin‐7‐amide.INTRODUCTIONThe black tiger shrimp Penaeus monodon is an economically important species of shrimp in Thailand that highly contributes to the value of seafood exports each year. The constant expansion of global shrimp demand has led to increases in stocking farms and a need for quality nauplii. Production of nauplii in hatcheries hinges on the high reproductive performance of broodstocks and high rates of fertilization. While artificial insemination in this species has been used to facilitate fertilization and breeding, the success of the technique is still dependent on sperm quality. In contrast to mammalian sperm, sperm quality in P. monodon has been difficult to assess due to their non‐motile nature and the lack of reliable fertility markers.Testicular spermatozoa generally lack fertilizing ability because they require activation by maturation factors that are secreted into the spermatic tract as well as in the female thelycum. For example, interactions of mammalian sperm with maturation or decapacitation factors in seminal vesicle fluid prevent premature capacitation and acrosome reaction; these are reversed in the oviduct, where sperm are activated by capacitation and undergo numerous additional changes (Fraser, ; Ni et al., ; Nixon

Journal

Molecular Reproduction & DevelopmentWiley

Published: Jan 1, 2018

Keywords: ; ;

References

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