Transcription‐coupled nucleotide excision repair (TC‐NER) is a subpathway of nucleotide excision repair that efficiently removes transcription‐blocking DNA damage from the transcribed strands of active genes. UVSSA is a causative gene for UV‐sensitive syndrome (UVSS), which is an autosomal recessive disorder characterized by hypersensitivity to UV light and deficiency in TC‐NER. UV‐stimulated scaffold protein A (UVSSA), the product of UVSSA, forms a complex with ubiquitin‐specific peptidase 7 (USP7) and is stabilized by interaction with USP7. The central region of UVSSA, which contains the tumor necrosis factor receptor‐associated factor (TRAF)‐binding motif, is required for the interaction with the N‐terminal TRAF domain of USP7. Here, we showed that UVSSA is mono‐ubiquitinated in vitro and identified a lysine residue (Lys414) in UVSSA as the target of ubiquitination. The deubiquitination activity of USP7 was inhibited by the ubiquitin‐conjugating enzyme UbcH6. Lys414 was also modified by poly‐ubiquitin chains in vivo. UVSSA deficient in the interaction with USP7 is ubiquitinated and degraded by the proteasome, and the degradation leads to deficiency in TC‐NER. The substitution of Lys414 by Arg of UVSSA inhibited its degradation and thereby suppressed the deficiency in TC‐NER.
Febs Journal – Wiley
Published: Jan 1, 2018
Keywords: ; ; ; ;
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera