Induction of micronuclei in murine lymphocytes by morphine

Induction of micronuclei in murine lymphocytes by morphine Although individuals who abuse drugs are prone to an increased risk of malignancy, the mutagenic and carcinogenic potential of these agents has received relatively little attention. We report here on the potential of morphine to induce micronuclei in murine lymphocytes. Following a single intraperitoneal injection of 20 mg/kg morphine, the frequency of micronucleated binuclear (cytochalasin‐blocked) murine T‐ and B‐splenocytes was elevated from 1 2–36 hr after treatment. The maximum frequencies seen 24 hr after injection were 6.3– and 4.9–fold greater than the respective controls. A dose‐dependent induction of micronuclei was observed from 5–20 mg/kg morphine, with no further increases in frequency produced by higher doses. In contrast, incubation of mitogen‐stimulated splenocytes with 10‐7‐10‐4 M morphine in vitro produced no change in frequency of micronucleated cells relative to controls. Treatment with the narcotic antagonist naloxone (5 mg/kg) alone had no effect on the frequency of micronuclei, but reduced the clasto‐genic response of a subsequently administered dose of morphine (20 mg/kg). Thus, in murine lymphocytes morphine indirectly produces genetic damage, which is at least in part opioid receptor‐mediated. © 1995 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Environmental and Molecular Mutagenesis Wiley

Induction of micronuclei in murine lymphocytes by morphine

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Publisher
Wiley
Copyright
Copyright © 1995 Wiley‐Liss, Inc., A Wiley Company
ISSN
0893-6692
eISSN
1098-2280
DOI
10.1002/em.2850250403
Publisher site
See Article on Publisher Site

Abstract

Although individuals who abuse drugs are prone to an increased risk of malignancy, the mutagenic and carcinogenic potential of these agents has received relatively little attention. We report here on the potential of morphine to induce micronuclei in murine lymphocytes. Following a single intraperitoneal injection of 20 mg/kg morphine, the frequency of micronucleated binuclear (cytochalasin‐blocked) murine T‐ and B‐splenocytes was elevated from 1 2–36 hr after treatment. The maximum frequencies seen 24 hr after injection were 6.3– and 4.9–fold greater than the respective controls. A dose‐dependent induction of micronuclei was observed from 5–20 mg/kg morphine, with no further increases in frequency produced by higher doses. In contrast, incubation of mitogen‐stimulated splenocytes with 10‐7‐10‐4 M morphine in vitro produced no change in frequency of micronucleated cells relative to controls. Treatment with the narcotic antagonist naloxone (5 mg/kg) alone had no effect on the frequency of micronuclei, but reduced the clasto‐genic response of a subsequently administered dose of morphine (20 mg/kg). Thus, in murine lymphocytes morphine indirectly produces genetic damage, which is at least in part opioid receptor‐mediated. © 1995 Wiley‐Liss, Inc.

Journal

Environmental and Molecular MutagenesisWiley

Published: Jan 1, 1995

References

  • Identification of aneuploidy‐inducing agents using cytokinesis‐blocked human lymphocytes and an antikinetochore antibody
    Eastmond, Eastmond; Tucker, Tucker
  • A modified mouse peripheral blood lymphocyte culture system for cytogenetic analysis
    Erexson, Erexson; Kligerman, Kligerman
  • Micronuclei as an index of cytogenetic damage: Past, present, and future
    Heddle, Heddle; Cimino, Cimino; Hayashi, Hayashi; Romagna, Romagna; Shelby, Shelby; Tucker, Tucker; Vanparys, Vanparys; MacGregor, MacGregor
  • Detection of opiate‐enhanced increases in DNA damage, HPRT mutants, and the mutation frequency in human HUT‐78 cells
    Shafer, Shafer; Xie, Xie; Falek, Falek
  • Opioid peptides and opioid receptors in cells of the immune system
    Sibinga, Sibinga; Goldstein, Goldstein

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