Inducible nitric oxide synthase and nitric oxide production by oligodendrocytes

Inducible nitric oxide synthase and nitric oxide production by oligodendrocytes It has been previously demonstrated that microglia and astrocytes produce micromolar amounts of nitric oxide in vitro. In this study, we demonstrate that primary rat oligodendrocytes can be stimulated to produce iNOS mRNA as detected by Northern blot and in situ hybridization analysis and a 131‐kDa iNOS protein by Western blot analysis; protein was also detected in cells by single‐ and double‐label immunohistochemistry for iNOS and the oligodendrocyte‐specific marker CNPase. NO/NOS are produced as a consequence of activation of the gene encoding the inducible nitric oxide synthase as determined by inhibition with actinomycin D and cyclohexamide. The iNOS is functional, leading to calcium/calmodulin‐independent NO production in these in vitro cultures. J. Neurosci. Res. 48:372–384, 1997. © 1997 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Research Wiley

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Publisher
Wiley
Copyright
Copyright © 1997 Wiley‐Liss, Inc.
ISSN
0360-4012
eISSN
1097-4547
DOI
10.1002/(SICI)1097-4547(19970515)48:4<372::AID-JNR9>3.0.CO;2-8
Publisher site
See Article on Publisher Site

Abstract

It has been previously demonstrated that microglia and astrocytes produce micromolar amounts of nitric oxide in vitro. In this study, we demonstrate that primary rat oligodendrocytes can be stimulated to produce iNOS mRNA as detected by Northern blot and in situ hybridization analysis and a 131‐kDa iNOS protein by Western blot analysis; protein was also detected in cells by single‐ and double‐label immunohistochemistry for iNOS and the oligodendrocyte‐specific marker CNPase. NO/NOS are produced as a consequence of activation of the gene encoding the inducible nitric oxide synthase as determined by inhibition with actinomycin D and cyclohexamide. The iNOS is functional, leading to calcium/calmodulin‐independent NO production in these in vitro cultures. J. Neurosci. Res. 48:372–384, 1997. © 1997 Wiley‐Liss, Inc.

Journal

Journal of Neuroscience ResearchWiley

Published: May 15, 1997

References

  • Induction of nitric oxide synthase in demyelinating regions of multiple sclerosis brains
    Bǒ, Bǒ; Dawson, Dawson; Wesselingh, Wesselingh; Mǐrk, Mǐrk; Choi, Choi; Kong, Kong; Hanley, Hanley; Trapp, Trapp
  • Cloning and expression of inducible nitric oxide synthase from rat astrocytes
    Galea, Galea; Reis, Reis; Feinstein, Feinstein
  • Secretion of nitrite by schwann cells and its effect on T‐cell activation in vitro
    Gold, Gold; Zielasek, Zielasek; Kiefer, Kiefer; Toyka, Toyka; Hartung, Hartung
  • Biosynthesis and metabolism of endothelium‐derived nitric oxide
    Ignarro, Ignarro
  • Effect of exogenous and endogenous nitric oxide on mitochondrial respiration of rat hepatocytes
    Stadler, Stadler; Billiar, Billiar; Curran, Curran; Stuehr, Stuehr; Ochoa, Ochoa; Simmons, Simmons

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