Increase of intracellular calcium induced by oxytocin in hypothalamic cultured astrocytes

Increase of intracellular calcium induced by oxytocin in hypothalamic cultured astrocytes A recent study demonstrated oxytocin (OT) receptors on hypothalamic cultured astrocytes (Di Scala‐Guenot and Strosser, 1992). The attempt in the present paper was to determine a possible intracellular calcium mobilization induced by OT receptor activation in these cells. Using the microspectrofluorimetric technique with fura‐2 as calcium indicator, brief applications of OT on single astrocytes induced a transient and reversible dose‐dependent increase of intracellular calcium concentration ((Ca2+)i) in most of the cells tested. In a few cells, OT application triggered intracellular calcium oscillations. Repetitive applications of OT generally produced a decreasing calcium signal, suggesting a desensitization of the receptor. OT‐induced calcium release was prevented by a prior or simultaneous application of an OT antagonist. The origin of the calcium mobilization was assessed during conditions where no extracellular calcium was available. Neither removal of extracellular calcium nor addition of a calcium channel blocker, cadmium 100 μM, in the bathing solution, did affect the calcium response to OT, demonstrating that release of intracellular calcium is solely involved in the OT‐induced (Ca2+)i increase. The OT‐induced calcium mobilization was abolished after thapsigargin application (100 nM). This indicates that the calcium response to OT application was principally associated with activation of the IP3‐sensitive calcium stores. Taken together these results demonstrate that OT receptors previously detected on hypothalamic cultured astrocytes are functional receptors which transduction pathways involve calcium mobilization from IP3‐sensitive stores. © 1994 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Glia Wiley

Increase of intracellular calcium induced by oxytocin in hypothalamic cultured astrocytes

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Publisher
Wiley
Copyright
Copyright © 1994 Wiley‐Liss, Inc.
ISSN
0894-1491
eISSN
1098-1136
DOI
10.1002/glia.440110308
pmid
7960031
Publisher site
See Article on Publisher Site

Abstract

A recent study demonstrated oxytocin (OT) receptors on hypothalamic cultured astrocytes (Di Scala‐Guenot and Strosser, 1992). The attempt in the present paper was to determine a possible intracellular calcium mobilization induced by OT receptor activation in these cells. Using the microspectrofluorimetric technique with fura‐2 as calcium indicator, brief applications of OT on single astrocytes induced a transient and reversible dose‐dependent increase of intracellular calcium concentration ((Ca2+)i) in most of the cells tested. In a few cells, OT application triggered intracellular calcium oscillations. Repetitive applications of OT generally produced a decreasing calcium signal, suggesting a desensitization of the receptor. OT‐induced calcium release was prevented by a prior or simultaneous application of an OT antagonist. The origin of the calcium mobilization was assessed during conditions where no extracellular calcium was available. Neither removal of extracellular calcium nor addition of a calcium channel blocker, cadmium 100 μM, in the bathing solution, did affect the calcium response to OT, demonstrating that release of intracellular calcium is solely involved in the OT‐induced (Ca2+)i increase. The OT‐induced calcium mobilization was abolished after thapsigargin application (100 nM). This indicates that the calcium response to OT application was principally associated with activation of the IP3‐sensitive calcium stores. Taken together these results demonstrate that OT receptors previously detected on hypothalamic cultured astrocytes are functional receptors which transduction pathways involve calcium mobilization from IP3‐sensitive stores. © 1994 Wiley‐Liss, Inc.

Journal

GliaWiley

Published: Jul 1, 1994

References

  • Characterization of glucocorticoid type II receptors in neuronal and glial cultures from rat brain
    Chou, Chou; Luttge, Luttge; Sumners, Sumners
  • Spontaneous changes in intracellular calcium concentration in type I astrocytes from rat cerebral cortex in primary culture
    Fatatis, Fatatis; Russell, Russell
  • A patch‐clamp study of bovine chromaffin cells and their sensitivity to acetylcholine
    Fenwick, Fenwick; Marty, Marty; Neher, Neher
  • Electrophysiological evidence for facilitatory control of oxytocin neurones by oxytocin during suckling in the rat
    Freund‐Mercier, Freund‐Mercier; Richard, Richard
  • Paraventricular and supraoptic bursting oxytocin cells in rat are locally regulated by oxytocin and functionally related
    Moos, Moos; Richard, Richard
  • Localization of oxytocin‐binding sites in the thoracic and upper lumbar spinal cord of the adult and postnatal rat: An histoautoradiographic study
    Reiter, Reiter; Kremarik, Kremarik; Freund‐Mercier, Freund‐Mercier; Stoeckel, Stoeckel; Desaulles, Desaulles; Feltz, Feltz
  • Lactation‐associated redistribution of the glial fibrillary acidic protein within the supraoptic nucleus
    Salm, Salm; Smithson, Smithson; Hatton, Hatton

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