Inactivation of human T‐cell lymphotropic virus, type III by heat, chemicals, and irradiation

Inactivation of human T‐cell lymphotropic virus, type III by heat, chemicals, and irradiation Abstract: Infectivity of human T‐cell lymphotropic virus, Type III (HTLV‐III) was inactivated by heat more rapidly if in liquid medium than if lyophilized and more rapidly at 60° than 56°C. When HTLV‐III was added to factor VIII suspension, then lyophilized and heated at 60°C for 2 hours or longer there was elimination of 1 × 106 in vitro infectious units (IVIU) of virus. Much of the viral inactivation appeared to result from lyophilization. The application of water‐saturated chloroform to the lyophilized material containing virus also resulted in elimination of infectivity. HTLV‐III was efficiently inactivated by formalin, beta‐propiolactone, ethyl ether, detergent, and ultraviolet light plus psoralen. The results are reassuring regarding the potential safety of various biological products. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Transfusion Wiley

Inactivation of human T‐cell lymphotropic virus, type III by heat, chemicals, and irradiation

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Abstract

Abstract: Infectivity of human T‐cell lymphotropic virus, Type III (HTLV‐III) was inactivated by heat more rapidly if in liquid medium than if lyophilized and more rapidly at 60° than 56°C. When HTLV‐III was added to factor VIII suspension, then lyophilized and heated at 60°C for 2 hours or longer there was elimination of 1 × 106 in vitro infectious units (IVIU) of virus. Much of the viral inactivation appeared to result from lyophilization. The application of water‐saturated chloroform to the lyophilized material containing virus also resulted in elimination of infectivity. HTLV‐III was efficiently inactivated by formalin, beta‐propiolactone, ethyl ether, detergent, and ultraviolet light plus psoralen. The results are reassuring regarding the potential safety of various biological products.

Journal

TransfusionWiley

Published: Sep 10, 1986

References

  • Evaluation of two viral inactivation methods for the preparation of safer factor VIII and factor IX concentrates
    Heldebrant, Heldebrant; Gomperts, Gomperts; Kasper, Kasper; McDougal, McDougal; Friedman, Friedman; Hwang, Hwang; Muchmore, Muchmore; Jordan, Jordan; Miller, Miller; Sergis‐Davenport, Sergis‐Davenport; Lam, Lam
  • Inactivation of viruses in labile blood derivatives. I. Disruption of lipid‐enveloped viruses by tri (N‐butyl) phosphate detergent combinations
    Horowitz, Horowitz; Wiebe, Wiebe; Lipin, Lipin; Stryker, Stryker
  • Inactivation of viruses in labile blood derivatives. II. Physical methods
    Horowitz, Horowitz; Wiebe, Wiebe; Lipin, Lipin; Vandersande, Vandersande; Stryker, Stryker

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