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In this issue: Biotechnology Journal 1/2006

In this issue: Biotechnology Journal 1/2006 Biotechnology Biotechnol. J. 2006, 1, 6 Journal In this issue Using budding yeast to screen for anti-prion drugs Tribouillard et al. Prions are misfolded proteins capable of propagating their altered conformation, commonly considered as the causative agents of some fatal neurodegenerative dis- eases, for which currently no treatment is available. A paper in this issue describes new developments of a novel yeast-based drug screening method, including the use of three different mammalian cell-based assays as secondary screens to unambigu- ously define compounds to be tested in vivo in mouse models for prion-based dis- eases. Confirming previous observations, most of the compounds isolated so far turned out to be also active in promoting mammalian prion clearance. This strongly suggests that biochemical pathways controlling prion formation and/or mainte- nance are conserved from yeast to human and thus amenable to biochemical and genetic analysis, and to pharmacological modulation. In addition, the yeast method could serve as a basis to isolate drugs potentially active against other diseases involving amyloid fibers, like Huntington’s, Parkinson’s or Alzheimer’s disease. ... 58 Electric field driven jetting: an emerging approach for processing living cells Jayasinghe et al. Novel techniques are required for processing suspensions of living cellular materi- als for the rapidly evolving fields in nano- and micro-biotechnology. This paper reports for the first time the ability to process living cellular materials by means of electrified jets at electric field strengths of up to 2 kV/mm. Electrohydrodynamic jet- ting (EHDJ) is a phenomenon where a multi-phase liquid is passed through a nee- dle at a controlled flow rate, where it undergoes charging by being exposed to a high intensity electric field. EHDJ can process concentrated suspensions from which droplets in the order of nanometers to a few micrometers can be generated. When bio-suspensions containing living human Jurkat cells are processed by this method, cells prove not to be harmed in any way by these intense electric fields. The authors hence extend this novel, economical and versatile approach for processing living cells for deposition. .................................................................................................... 86 The first automated synthesis of ferrocene-labelled phosphorothioate DNA probe: a new potential tool for the fabrication of DNA microarrays Brisset et al. Extensive research focusses on the (faster) transduction of DNA hybridization for applications in for example clinical diagnostics, environmental protection or food quality control. Electrochemical methods receive particular attention due to their + + cDNA cDNA denaturation denaturation high sensitivity, easy instrumentation, low production cost and compatibility to make small devices. In this article, Brisset et al. describe the synthesis and the char- acterization of the first electroactive ferrocene-labelled oligonucleotide phosphoroth- ioate probe (ODN-Fc-Ps) obtained by automated synthesis. After grafting this probe on a gold electrode, the DNA hybridization with a complementary strand is moni- tored by the change in electrochemical response of the ferrocene moiety. The ODN- Fc-Ps probe presents a promising basis for simple and highly selective sensing sys- tems.............................................................................................................................. 95 6 © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Journal Wiley

In this issue: Biotechnology Journal 1/2006

Biotechnology Journal , Volume 1 (1) – Jan 1, 2006
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Publisher
Wiley
Copyright
Copyright © 2006 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1860-6768
eISSN
1860-7314
DOI
10.1002/biot.200690012
Publisher site
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Abstract

Biotechnology Biotechnol. J. 2006, 1, 6 Journal In this issue Using budding yeast to screen for anti-prion drugs Tribouillard et al. Prions are misfolded proteins capable of propagating their altered conformation, commonly considered as the causative agents of some fatal neurodegenerative dis- eases, for which currently no treatment is available. A paper in this issue describes new developments of a novel yeast-based drug screening method, including the use of three different mammalian cell-based assays as secondary screens to unambigu- ously define compounds to be tested in vivo in mouse models for prion-based dis- eases. Confirming previous observations, most of the compounds isolated so far turned out to be also active in promoting mammalian prion clearance. This strongly suggests that biochemical pathways controlling prion formation and/or mainte- nance are conserved from yeast to human and thus amenable to biochemical and genetic analysis, and to pharmacological modulation. In addition, the yeast method could serve as a basis to isolate drugs potentially active against other diseases involving amyloid fibers, like Huntington’s, Parkinson’s or Alzheimer’s disease. ... 58 Electric field driven jetting: an emerging approach for processing living cells Jayasinghe et al. Novel techniques are required for processing suspensions of living cellular materi- als for the rapidly evolving fields in nano- and micro-biotechnology. This paper reports for the first time the ability to process living cellular materials by means of electrified jets at electric field strengths of up to 2 kV/mm. Electrohydrodynamic jet- ting (EHDJ) is a phenomenon where a multi-phase liquid is passed through a nee- dle at a controlled flow rate, where it undergoes charging by being exposed to a high intensity electric field. EHDJ can process concentrated suspensions from which droplets in the order of nanometers to a few micrometers can be generated. When bio-suspensions containing living human Jurkat cells are processed by this method, cells prove not to be harmed in any way by these intense electric fields. The authors hence extend this novel, economical and versatile approach for processing living cells for deposition. .................................................................................................... 86 The first automated synthesis of ferrocene-labelled phosphorothioate DNA probe: a new potential tool for the fabrication of DNA microarrays Brisset et al. Extensive research focusses on the (faster) transduction of DNA hybridization for applications in for example clinical diagnostics, environmental protection or food quality control. Electrochemical methods receive particular attention due to their + + cDNA cDNA denaturation denaturation high sensitivity, easy instrumentation, low production cost and compatibility to make small devices. In this article, Brisset et al. describe the synthesis and the char- acterization of the first electroactive ferrocene-labelled oligonucleotide phosphoroth- ioate probe (ODN-Fc-Ps) obtained by automated synthesis. After grafting this probe on a gold electrode, the DNA hybridization with a complementary strand is moni- tored by the change in electrochemical response of the ferrocene moiety. The ODN- Fc-Ps probe presents a promising basis for simple and highly selective sensing sys- tems.............................................................................................................................. 95 6 © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

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Biotechnology Journal – Wiley

Published: Jan 1, 2006

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