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D. Marquardt (1963)
An Algorithm for Least-Squares Estimation of Nonlinear ParametersJournal of The Society for Industrial and Applied Mathematics, 11
J. P. Chandler (1983)
STEPIT. Quantum Chemistry Program Exchange
B. Schutte, M. Reynders, C. Assche, P. Hupperets, F. Bosman, G. Blijham (1987)
An improved method for the immunocytochemical detection of bromodeoxyuridine labeled nuclei using flow cytometry.Cytometry, 8 4
A. Begg, L. Moonen, I. Hofland, M. Dessing, Harry Bartelink (1988)
Human tumour cell kinetics using a monoclonal antibody against iododeoxyuridine: intratumour sampling variations.Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, 11 4
R. White, M. Meistrich (1986)
A comment on "A method to measure the duration of DNA synthesis and the potential doubling time from a single sample".Cytometry, 7 5
A. Riccardi, M. Danova, G. Wilson, G. Ucci, P. Dormer, G. Mazzini, S. Brugnatelli, M. Girino, Nicholas McNally, E. Ascari (1988)
Cell kinetics in human malignancies studied with in vivo administration of bromodeoxyuridine and flow cytometry.Cancer research, 48 21
A. Begg, N. McNally, D. Shrieve, H. Kärche (1985)
A method to measure the duration of DNA synthesis and the potential doubling time from a single sample.Cytometry, 6 6
F. Dolbeare, H. Gratzner, M. Pallavicini, J. Gray (1983)
Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine.Proceedings of the National Academy of Sciences of the United States of America, 80 18
G. Steel (1977)
Growth kinetics of tumours : cell population kinetics in relation to the growth and treatment of cancer
Relative movement methods use the timed progression of the mean fluorescence of cells which have heen labeled with monoclonal antibodies against bromodeoxyuridine and displayed with bivariate flow cytometry according to DNA and label content to compute duration of DNA synthesis, Ts. The relative movement is the difference of the mean DNA fluorescence of the labeled undivided cells from the G1 channel relative to the difference between the G1 and G2M channels. In this communication, we show how to extend this method to compute the potential doubling time, Tpot, the time required for a population of cells to double, given quiescent cells but no cell loss. A quantity vis introduced that is a function of the fraction of labeled divided cells and the fraction of labeled undivided cells. We show that v is independent of time and is equal to In(2)T8/Tpot so that Tpot (equal to In(2)T8/v) can be directly found from the information available in computing the relative movement. The method is applied to Chinese hamster ovary cells to demonstrate its utility.
Cytometry Part A – Wiley
Published: Jan 1, 1990
Keywords: ; ; ;
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