Improved bacterial identification directly from urine samples with matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Improved bacterial identification directly from urine samples with matrix‐assisted laser... INTRODUCTIONThe clinical use of matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) has spread as a new method of bacterial identification due to the simplicity and speed of the procedure. Reportedly, MALDI‐TOF MS can identify bacteria directly from culture‐positive blood samples. Rapid identification of causative bacteria in common infectious diseases such as upper respiratory tract infection (RTI) or urinary tract infection (UTI) are beneficial for patients. In order to apply MALDI‐TOF MS for routine clinical diagnosis, direct comparison between MALDI‐TOF MS and conventional cultures is required, especially in complicated UTI cases where it is not easy to assume the causative bacteria.Several institutions have applied this method in the clinic for bacteremia but comparatively few have adopted it for UTI. This method can detect bacteria in blood, but contamination of urine by more than one kind of bacteria, cluster formation, and the smaller size of Gram‐positive bacteria have made MALDI‐TOF MS identification of Gram‐positive bacteria in urine difficult, requiring some additional procedure for separating bacterial aggregation. There are no standardized protocols for sample preparation for identifying bacteria from urine culture, which may include several kinds of bacteria, causative or not. In this study, we especially focused on Gram‐positive bacteria, which http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Laboratory Analysis Wiley

Improved bacterial identification directly from urine samples with matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 Wiley Periodicals, Inc.
ISSN
0887-8013
eISSN
1098-2825
D.O.I.
10.1002/jcla.22301
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONThe clinical use of matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) has spread as a new method of bacterial identification due to the simplicity and speed of the procedure. Reportedly, MALDI‐TOF MS can identify bacteria directly from culture‐positive blood samples. Rapid identification of causative bacteria in common infectious diseases such as upper respiratory tract infection (RTI) or urinary tract infection (UTI) are beneficial for patients. In order to apply MALDI‐TOF MS for routine clinical diagnosis, direct comparison between MALDI‐TOF MS and conventional cultures is required, especially in complicated UTI cases where it is not easy to assume the causative bacteria.Several institutions have applied this method in the clinic for bacteremia but comparatively few have adopted it for UTI. This method can detect bacteria in blood, but contamination of urine by more than one kind of bacteria, cluster formation, and the smaller size of Gram‐positive bacteria have made MALDI‐TOF MS identification of Gram‐positive bacteria in urine difficult, requiring some additional procedure for separating bacterial aggregation. There are no standardized protocols for sample preparation for identifying bacteria from urine culture, which may include several kinds of bacteria, causative or not. In this study, we especially focused on Gram‐positive bacteria, which

Journal

Journal of Clinical Laboratory AnalysisWiley

Published: Jan 1, 2018

Keywords: ; ; ; ; ;

References

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