The standardization is described of an immunofluorescent microphotometric method that offers a solution for the problems generally encountered in immunofluorescence procedures on platelets. Fluorescein isothiocyanate (FITC) conjugated .anti‐immunoglobulin serum and anti‐IgG serum were used. Sera, containing isoor hetero‐antibodies against platelets that had given positive reactions with one or more of the conventional serological techniques, were used as positive controls. The method described proved to have a good reproducibility. Significant differences in fluorescence were obtained using the above‐mentioned positive sera and normal control sera. It was found that platelets stored in sodium‐azide could be used as antigen. A number of variables that may influence the difference in fluorescence obtained with positive and normal sera were studied.
Scandinavian Journal of Immunology – Wiley
Published: May 1, 1973
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