Immunofluorescence Microphotometry for the Detection of Platelet Antibodies. I. Standardization of the Method

Immunofluorescence Microphotometry for the Detection of Platelet Antibodies. I. Standardization... The standardization is described of an immunofluorescent microphotometric method that offers a solution for the problems generally encountered in immunofluorescence procedures on platelets. Fluorescein isothiocyanate (FITC) conjugated .anti‐immunoglobulin serum and anti‐IgG serum were used. Sera, containing isoor hetero‐antibodies against platelets that had given positive reactions with one or more of the conventional serological techniques, were used as positive controls. The method described proved to have a good reproducibility. Significant differences in fluorescence were obtained using the above‐mentioned positive sera and normal control sera. It was found that platelets stored in sodium‐azide could be used as antigen. A number of variables that may influence the difference in fluorescence obtained with positive and normal sera were studied. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Scandinavian Journal of Immunology Wiley

Immunofluorescence Microphotometry for the Detection of Platelet Antibodies. I. Standardization of the Method

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Publisher
Wiley
Copyright
Copyright © 1973 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0300-9475
eISSN
1365-3083
DOI
10.1111/j.1365-3083.1973.tb02032.x
Publisher site
See Article on Publisher Site

Abstract

The standardization is described of an immunofluorescent microphotometric method that offers a solution for the problems generally encountered in immunofluorescence procedures on platelets. Fluorescein isothiocyanate (FITC) conjugated .anti‐immunoglobulin serum and anti‐IgG serum were used. Sera, containing isoor hetero‐antibodies against platelets that had given positive reactions with one or more of the conventional serological techniques, were used as positive controls. The method described proved to have a good reproducibility. Significant differences in fluorescence were obtained using the above‐mentioned positive sera and normal control sera. It was found that platelets stored in sodium‐azide could be used as antigen. A number of variables that may influence the difference in fluorescence obtained with positive and normal sera were studied.

Journal

Scandinavian Journal of ImmunologyWiley

Published: May 1, 1973

References

  • Influence of cryoprofibrin on the antiglobulin consumption test with platelets
    Weerdt, Weerdt; Vreeken, Vreeken

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