Immunoelectron microscope demonstration of the basement membrane components laminin and type IV collagen in the dermal cylindroma

Immunoelectron microscope demonstration of the basement membrane components laminin and type IV... Specific antisera to the human laminin P1 fragment and the 7S domain of type IV collagen were used to investigate the ultrastructural location of these main basement membrane (BM) components in a dermal cylindroma. Thick frozen sections were treated by the immunoperoxidase method, postfixed, embedded in epon and then sectioned for electron microscopic examination. Laminin and type IV collagen were detected in all layers of the cylindroma BM, together with non‐specifically stained, diffusely distributed fibrillar structures, possible microfibrils and anchoring fibrils or residual stromal collagen fibres. The mixed presence of the BM components indicates that these substances codistribute rather than occurring as separate layers even when forming neoplastic basement membranes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Pathology Wiley

Immunoelectron microscope demonstration of the basement membrane components laminin and type IV collagen in the dermal cylindroma

The Journal of Pathology, Volume 147 (2) – Oct 1, 1985

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Publisher
Wiley
Copyright
Copyright © 1985 John Wiley & Sons, Ltd.
ISSN
0022-3417
eISSN
1096-9896
DOI
10.1002/path.1711470204
pmid
2999366
Publisher site
See Article on Publisher Site

Abstract

Specific antisera to the human laminin P1 fragment and the 7S domain of type IV collagen were used to investigate the ultrastructural location of these main basement membrane (BM) components in a dermal cylindroma. Thick frozen sections were treated by the immunoperoxidase method, postfixed, embedded in epon and then sectioned for electron microscopic examination. Laminin and type IV collagen were detected in all layers of the cylindroma BM, together with non‐specifically stained, diffusely distributed fibrillar structures, possible microfibrils and anchoring fibrils or residual stromal collagen fibres. The mixed presence of the BM components indicates that these substances codistribute rather than occurring as separate layers even when forming neoplastic basement membranes.

Journal

The Journal of PathologyWiley

Published: Oct 1, 1985

References

  • Laminin, fibronectin and collagen in synaptic and extrasynaptic portions of muscle fiber basement membrane
    Sanes, Sanes
  • Localization of type IV collagen, laminin, heparan sulfate proteoglycan and fibronectin to the basal lamina of basement membranes
    Laurie, Laurie; Leblond, Leblond; Martin, Martin
  • Ultrastructural localization of fibronectin and laminin in the basement membranes of the murine kidney
    Madri, Madri; Roll, Roll; Furthmayr, Furthmayr; Foidart, Foidart
  • A specific antigenic defect of the basement membrane is found in basal cell carcinoma but not in other epidermal tumors
    Stanley, Stanley; Beckwith, Beckwith; Fuller, Fuller; Katz, Katz
  • Monoclonal antibodies to laminin reveal the heterogeneity of basement membranes in the developing and adult mouse tissues
    Wan, Wan; Wu, Wu; Chung, Chung; Damjanov, Damjanov
  • Cutaneous immunoelectron microscopy
    Holden, Holden; MacDonald, MacDonald
  • Ultrastructural study of cylindroma (Poncet‐Spiegler tumor)
    Reynes, Reynes; Puissant, Puissant; Delanoe, Delanoe; Noury‐Dupperrat, Noury‐Dupperrat; Saurat, Saurat

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