Identification of novel intergenic repetitive units in a mycobacterial two‐component system operon

Identification of novel intergenic repetitive units in a mycobacterial two‐component system operon Mycobacterial interspersed repetitive units (MIRUs), a novel class of repeated sequences, were identified within the intercistronic region of an operon coding for a mycobacterial two‐component system, named senX3‐regX3. Southern blot analysis and homology searches revealed the presence of several homologous sequences in intergenic regions dispersed throughout the genomes of Mycobacterium bovis BCG, Mycobacterium tuberculosis and Mycobacterium leprae. These could be grouped into three major families, containing elements of 77–101 bp, 46–53 bp and 58–101 bp. Based on the available mycobacterial sequences, the total number of MIRUs is estimated to be about 40–50 per genome. Similar to previously identified small repetitive sequences, the MIRUs of the two‐component operon are transcribed on a polycistronic mRNA. Unlike previously identified small repetitive sequences, however, MIRUs do not contain dyad symmetries, comprise small open reading frames (ORFs) whose extremities overlap those of the contiguous ORFs and are oriented in the same translational direction as those of the adjacent genes. Analyses of the sequences at the insertion sites suggest that MIRUs disseminate by transposition into DTGA sites involved in translational coupling in polycistronic operons. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Microbiology Wiley

Identification of novel intergenic repetitive units in a mycobacterial two‐component system operon

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Publisher
Wiley
Copyright
Blackwell Science Ltd, Oxford
ISSN
0950-382X
eISSN
1365-2958
DOI
10.1046/j.1365-2958.1997.6361999.x
Publisher site
See Article on Publisher Site

Abstract

Mycobacterial interspersed repetitive units (MIRUs), a novel class of repeated sequences, were identified within the intercistronic region of an operon coding for a mycobacterial two‐component system, named senX3‐regX3. Southern blot analysis and homology searches revealed the presence of several homologous sequences in intergenic regions dispersed throughout the genomes of Mycobacterium bovis BCG, Mycobacterium tuberculosis and Mycobacterium leprae. These could be grouped into three major families, containing elements of 77–101 bp, 46–53 bp and 58–101 bp. Based on the available mycobacterial sequences, the total number of MIRUs is estimated to be about 40–50 per genome. Similar to previously identified small repetitive sequences, the MIRUs of the two‐component operon are transcribed on a polycistronic mRNA. Unlike previously identified small repetitive sequences, however, MIRUs do not contain dyad symmetries, comprise small open reading frames (ORFs) whose extremities overlap those of the contiguous ORFs and are oriented in the same translational direction as those of the adjacent genes. Analyses of the sequences at the insertion sites suggest that MIRUs disseminate by transposition into DTGA sites involved in translational coupling in polycistronic operons.

Journal

Molecular MicrobiologyWiley

Published: Dec 1, 1997

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