Identification of genes co‐upregulated with Arc during BDNF‐induced long‐term potentiation in adult rat dentate gyrus in vivo

Identification of genes co‐upregulated with Arc during BDNF‐induced long‐term potentiation... Brain‐derived neurotrophic factor (BDNF) is a critical regulator of transcription‐dependent adaptive neuronal responses, such as long‐term potentiation (LTP). Brief infusion of BDNF into the dentate gyrus of adult anesthetized rats triggers stable LTP at medial perforant path‐granule synapses that is transcription‐dependent and requires induction of the immediate early gene Arc. Rather than acting alone, Arc is likely to be part of a larger BDNF‐induced transcriptional program. Here, we used cDNA microarray expression profiling to search for genes co‐upregulated with Arc 3 h after BDNF‐LTP induction. Of nine cDNAs encoding for known genes and up‐regulated more than four‐fold, we selected five genes, Narp, neuritin, ADP‐ribosylation factor‐like protein‐4 (ARL4L), TGF‐β‐induced immediate early gene‐1 (TIEG1) and CARP, for further validation. Real‐time PCR confirmed robust up‐regulation of these genes in an independent set of BDNF‐LTP experiments, whereas infusion of the control protein cytochrome C had no effect. In situ hybridization histochemistry further revealed up‐regulation of all five genes in somata of post‐synaptic granule cells following both BDNF‐LTP and high‐frequency stimulation‐induced LTP. While Arc synthesis is critical for local actin polymerization and stable LTP formation, several of the co‐upregulated genes have known functions in excitatory synaptogenesis, axon guidance and glutamate receptor clustering. These results provide novel insight into gene expression responses underlying BDNF‐induced synaptic consolidation in the adult brain in vivo. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Neuroscience Wiley

Identification of genes co‐upregulated with Arc during BDNF‐induced long‐term potentiation in adult rat dentate gyrus in vivo

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Publisher
Wiley
Copyright
Copyright © 2006 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0953-816X
eISSN
1460-9568
D.O.I.
10.1111/j.1460-9568.2006.04687.x
Publisher site
See Article on Publisher Site

Abstract

Brain‐derived neurotrophic factor (BDNF) is a critical regulator of transcription‐dependent adaptive neuronal responses, such as long‐term potentiation (LTP). Brief infusion of BDNF into the dentate gyrus of adult anesthetized rats triggers stable LTP at medial perforant path‐granule synapses that is transcription‐dependent and requires induction of the immediate early gene Arc. Rather than acting alone, Arc is likely to be part of a larger BDNF‐induced transcriptional program. Here, we used cDNA microarray expression profiling to search for genes co‐upregulated with Arc 3 h after BDNF‐LTP induction. Of nine cDNAs encoding for known genes and up‐regulated more than four‐fold, we selected five genes, Narp, neuritin, ADP‐ribosylation factor‐like protein‐4 (ARL4L), TGF‐β‐induced immediate early gene‐1 (TIEG1) and CARP, for further validation. Real‐time PCR confirmed robust up‐regulation of these genes in an independent set of BDNF‐LTP experiments, whereas infusion of the control protein cytochrome C had no effect. In situ hybridization histochemistry further revealed up‐regulation of all five genes in somata of post‐synaptic granule cells following both BDNF‐LTP and high‐frequency stimulation‐induced LTP. While Arc synthesis is critical for local actin polymerization and stable LTP formation, several of the co‐upregulated genes have known functions in excitatory synaptogenesis, axon guidance and glutamate receptor clustering. These results provide novel insight into gene expression responses underlying BDNF‐induced synaptic consolidation in the adult brain in vivo.

Journal

European Journal of NeuroscienceWiley

Published: Mar 1, 2006

References

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    Bramham, Bramham; Messaoudi, Messaoudi
  • BDNF‐induced LTP in dentate gyrus is impaired with age: analysis of changes in cell signaling events
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  • Synaptic secretion of BDNF after high‐frequency stimulation of glutamatergic synapses
    Hartmann, Hartmann; Heumann, Heumann; Lessmann, Lessmann
  • Hippocampal plasticity involves extensive gene induction and multiple cellular mechanisms
    Hevroni, Hevroni; Rattner, Rattner; Bundman, Bundman; Lederfein, Lederfein; Gabarah, Gabarah; Mangelus, Mangelus; Silverman, Silverman; Kedar, Kedar; Naor, Naor; Kornuc, Kornuc; Hanoch, Hanoch; Seger, Seger; Theill, Theill; Nedivi, Nedivi; Richter‐Levin, Richter‐Levin; Citri, Citri
  • Role of ARF4L in recycling between endosomes and the plasma membrane
    Katayama, Katayama; Imaizumi, Imaizumi; Yoneda, Yoneda; Taniguchi, Taniguchi; Honda, Honda; Manabe, Manabe; Hitomi, Hitomi; Oono, Oono; Baba, Baba; Miyata, Miyata; Matsuzaki, Matsuzaki; Takatsuji, Takatsuji; Tohyama, Tohyama
  • Acute intrahippocampal infusion of BDNF induces lasting potentiation of synaptic transmission in the rat dentate gyrus
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  • SHARPs: mammalian enhancer‐of‐split‐ and hairy‐related proteins coupled to neuronal stimulation
    Rossner, Rossner; Dorr, Dorr; Gass, Gass; Schwab, Schwab; Nave, Nave
  • Sequential changes in the synaptic structural profile following long‐term potentiation in the rat dentate gyrus. III. Long‐term maintenance phase
    Weeks, Weeks; Ivanco, Ivanco; LeBoutillier, LeBoutillier; Racine, Racine; Petit, Petit
  • Regulation of notch endosomal sorting and signaling by Drosophila Nedd4 family proteins
    Wilkin, Wilkin; Carbery, Carbery; Fostier, Fostier; Aslam, Aslam; Mazaleyrat, Mazaleyrat; Higgs, Higgs; Myat, Myat; Evans, Evans; Cornell, Cornell; Baron, Baron

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