Brain‐derived neurotrophic factor (BDNF) is a critical regulator of transcription‐dependent adaptive neuronal responses, such as long‐term potentiation (LTP). Brief infusion of BDNF into the dentate gyrus of adult anesthetized rats triggers stable LTP at medial perforant path‐granule synapses that is transcription‐dependent and requires induction of the immediate early gene Arc. Rather than acting alone, Arc is likely to be part of a larger BDNF‐induced transcriptional program. Here, we used cDNA microarray expression profiling to search for genes co‐upregulated with Arc 3 h after BDNF‐LTP induction. Of nine cDNAs encoding for known genes and up‐regulated more than four‐fold, we selected five genes, Narp, neuritin, ADP‐ribosylation factor‐like protein‐4 (ARL4L), TGF‐β‐induced immediate early gene‐1 (TIEG1) and CARP, for further validation. Real‐time PCR confirmed robust up‐regulation of these genes in an independent set of BDNF‐LTP experiments, whereas infusion of the control protein cytochrome C had no effect. In situ hybridization histochemistry further revealed up‐regulation of all five genes in somata of post‐synaptic granule cells following both BDNF‐LTP and high‐frequency stimulation‐induced LTP. While Arc synthesis is critical for local actin polymerization and stable LTP formation, several of the co‐upregulated genes have known functions in excitatory synaptogenesis, axon guidance and glutamate receptor clustering. These results provide novel insight into gene expression responses underlying BDNF‐induced synaptic consolidation in the adult brain in vivo.
European Journal of Neuroscience – Wiley
Published: Mar 1, 2006
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera