Identification of antioxidant and ACE‐inhibitory peptides in fermented milk

Identification of antioxidant and ACE‐inhibitory peptides in fermented milk The angiotensin‐converting enzyme (ACE)‐inhibitory activity and antioxidant properties of a commercial fermented milk from Europe were evaluated. This dairy product showed moderate ACE‐inhibitory activity and ABTS•+ radical‐scavenging capacity. The peptides from most active fractions collected by reverse phase high‐performance liquid chromatography (RP‐HPLC) were sequenced by RP‐HPLC–tandem mass spectrometry. This technique allowed rapid identification of peptides included in the most active fractions, and various potentially active peptides were recognised according to previous studies of structure–activity relationship. Three of the identified sequences had previously been described as potent ACE inhibitors. The structure of some sequences substantiated the presence of peptides with ACE‐inhibitory, antioxidant and immunomodulatory activities. Copyright © 2005 Society of Chemical Industry http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of the Science of Food and Agriculture Wiley

Identification of antioxidant and ACE‐inhibitory peptides in fermented milk

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Publisher
Wiley
Copyright
Copyright © 2005 Society of Chemical Industry
ISSN
0022-5142
eISSN
1097-0010
D.O.I.
10.1002/jsfa.2063
Publisher site
See Article on Publisher Site

Abstract

The angiotensin‐converting enzyme (ACE)‐inhibitory activity and antioxidant properties of a commercial fermented milk from Europe were evaluated. This dairy product showed moderate ACE‐inhibitory activity and ABTS•+ radical‐scavenging capacity. The peptides from most active fractions collected by reverse phase high‐performance liquid chromatography (RP‐HPLC) were sequenced by RP‐HPLC–tandem mass spectrometry. This technique allowed rapid identification of peptides included in the most active fractions, and various potentially active peptides were recognised according to previous studies of structure–activity relationship. Three of the identified sequences had previously been described as potent ACE inhibitors. The structure of some sequences substantiated the presence of peptides with ACE‐inhibitory, antioxidant and immunomodulatory activities. Copyright © 2005 Society of Chemical Industry

Journal

Journal of the Science of Food and AgricultureWiley

Published: Apr 30, 2005

References

  • Milk derived peptides and hypertension reduction
    Takano, Takano
  • Isolation and characterization of free radical scavenging activities peptides derived from casein
    Suetsuna, Suetsuna; Ukeda, Ukeda; Ochi, Ochi
  • Purification and characterization of antioxidative peptides from protein hydrolysate of lecithin‐free egg yolk
    Park, Park; Jung, Jung; Nam, Nam; Shahidi, Shahidi; Kim, Kim
  • Angiotensin I converting enzyme inhibitory peptides derived from bovine milk proteins
    Pihlanto‐Leppälä, Pihlanto‐Leppälä; Rokka, Rokka; Korhonen, Korhonen

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