Human skin in vivo has a higher skin barrier function than porcine skin ex vivo—comprehensive Raman microscopic study of the stratum corneum

Human skin in vivo has a higher skin barrier function than porcine skin ex vivo—comprehensive... Porcine skin is widely used as a human skin model in dermatology. For both, porcine stratum corneum (SC) ex vivo and human SC in vivo, the hydrogen bonding states of water, the secondary and tertiary structures of keratin, the natural moisturizing factor (NMF) concentrations and the intercellular lipids' (ICL) lateral organization are investigated depth‐dependently using confocal Raman microscopy. The SC depth profiles show that porcine SC ex vivo is characterized by lower hydrogen bonding states of water (10%‐30% SC depth), lower NMF concentration in the whole SC, more β‐sheet form of keratin (10%‐90% SC depth), more folded tertiary keratin structures (30%‐70% SC depth) and higher hexagonal lateral packing order of ICL (10%‐50% SC depth) compared to human SC in vivo. The results clearly show a higher value of skin barrier function of human SC in vivo than of porcine SC ex vivo. Thus, the human SC in vivo is less permeable for lipophilic and hydrophilic substances than porcine SC ex vivo. Considering the porcine SC as an ex vivo model of human SC in vivo, these findings should be taken into consideration. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biophotonics Wiley

Human skin in vivo has a higher skin barrier function than porcine skin ex vivo—comprehensive Raman microscopic study of the stratum corneum

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Publisher
Wiley
Copyright
© 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
ISSN
1864-063X
eISSN
1864-0648
D.O.I.
10.1002/jbio.201700355
Publisher site
See Article on Publisher Site

Abstract

Porcine skin is widely used as a human skin model in dermatology. For both, porcine stratum corneum (SC) ex vivo and human SC in vivo, the hydrogen bonding states of water, the secondary and tertiary structures of keratin, the natural moisturizing factor (NMF) concentrations and the intercellular lipids' (ICL) lateral organization are investigated depth‐dependently using confocal Raman microscopy. The SC depth profiles show that porcine SC ex vivo is characterized by lower hydrogen bonding states of water (10%‐30% SC depth), lower NMF concentration in the whole SC, more β‐sheet form of keratin (10%‐90% SC depth), more folded tertiary keratin structures (30%‐70% SC depth) and higher hexagonal lateral packing order of ICL (10%‐50% SC depth) compared to human SC in vivo. The results clearly show a higher value of skin barrier function of human SC in vivo than of porcine SC ex vivo. Thus, the human SC in vivo is less permeable for lipophilic and hydrophilic substances than porcine SC ex vivo. Considering the porcine SC as an ex vivo model of human SC in vivo, these findings should be taken into consideration.

Journal

Journal of BiophotonicsWiley

Published: Jan 1, 2018

Keywords: ; ; ; ; ; ; ;

References

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