How a phase image of a cell with nucleus refractive index smaller than that of the cytoplasm should look like?

How a phase image of a cell with nucleus refractive index smaller than that of the cytoplasm... In recent papers Steelman et al. (“Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies”) and Schürmann et al. (“Cell nuclei have lower refractive index and mass density than cytoplasm”) obtained quantitative phase images of whole cells of various types and corresponding isolated nuclei and concluded that the refractive index (RI) of the nucleus is significantly smaller than that of the cytoplasm. The comment shows that this conclusion and assumptions used in retrieving the RI necessarily imply a characteristic dip in the center of the whole‐cell phase images. This dip is not present in any of the phase images in the discussed papers, which is a strong argument against the conclusion of smaller nucleus RI. It is also discussed whether a different processing of the phase images can help to clarify this issue. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biophotonics Wiley

How a phase image of a cell with nucleus refractive index smaller than that of the cytoplasm should look like?

Loading next page...
 
/lp/wiley/how-a-phase-image-of-a-cell-with-nucleus-refractive-index-smaller-than-8tp0A0tSw9
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
© 2018 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
ISSN
1864-063X
eISSN
1864-0648
D.O.I.
10.1002/jbio.201800033
Publisher site
See Article on Publisher Site

Abstract

In recent papers Steelman et al. (“Is the nuclear refractive index lower than cytoplasm? Validation of phase measurements and implications for light scattering technologies”) and Schürmann et al. (“Cell nuclei have lower refractive index and mass density than cytoplasm”) obtained quantitative phase images of whole cells of various types and corresponding isolated nuclei and concluded that the refractive index (RI) of the nucleus is significantly smaller than that of the cytoplasm. The comment shows that this conclusion and assumptions used in retrieving the RI necessarily imply a characteristic dip in the center of the whole‐cell phase images. This dip is not present in any of the phase images in the discussed papers, which is a strong argument against the conclusion of smaller nucleus RI. It is also discussed whether a different processing of the phase images can help to clarify this issue.

Journal

Journal of BiophotonicsWiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off