Histamine Stimulation of Cyclic AMP Accumulation in Astrocyte‐Enriched and Neuronal Primary Cultures from Rat Brain

Histamine Stimulation of Cyclic AMP Accumulation in Astrocyte‐Enriched and Neuronal Primary... Abstract: Histamine stimulates cyclic AMP accumulation in astrocyte‐enriched and neuronal primary cultures from rat brain in the presence of the phosphodiesterase inhibitor isobutylmethylxanthine. The response in the astrocyte cultures (Emax= 304 ± 44% over basal, EC50= 43 ± 5 μM) was much higher than in neuronal cultures (Emax= 24 ±2%, EC50= 14 ± 7 μM). The histamine effect in astrocytes was competitively inhibited by the H2 antagonists cimetidine (Ki=1.1 ± 0.2 μM) and ranitidine (Ki‐ 46 ± 10 nM) but was insensitive to the H1 antagonist mepyramine (1 μM). The two selective H2 agonists impromidine and dimaprit behaved as partial agonists and showed relative potencies (139 and 0.5, respectively) consistent with an interaction with H2 receptors. The more selective H1 agonist 2‐thiazolylethylamine (0.01–1 mM) did not potentiate the response to impromidine (10 μM). Thus, in contrast to what is generally observed in intact cell preparations from brain, the histamine‐induced cyclic AMP accumulation in astroglial cells is mediated solely by H2 receptors. The small effect shown in neuronal cultures also appears to be mediated by H2 receptors. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Histamine Stimulation of Cyclic AMP Accumulation in Astrocyte‐Enriched and Neuronal Primary Cultures from Rat Brain

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Publisher
Wiley
Copyright
Copyright © 1990 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1990.tb04943.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Histamine stimulates cyclic AMP accumulation in astrocyte‐enriched and neuronal primary cultures from rat brain in the presence of the phosphodiesterase inhibitor isobutylmethylxanthine. The response in the astrocyte cultures (Emax= 304 ± 44% over basal, EC50= 43 ± 5 μM) was much higher than in neuronal cultures (Emax= 24 ±2%, EC50= 14 ± 7 μM). The histamine effect in astrocytes was competitively inhibited by the H2 antagonists cimetidine (Ki=1.1 ± 0.2 μM) and ranitidine (Ki‐ 46 ± 10 nM) but was insensitive to the H1 antagonist mepyramine (1 μM). The two selective H2 agonists impromidine and dimaprit behaved as partial agonists and showed relative potencies (139 and 0.5, respectively) consistent with an interaction with H2 receptors. The more selective H1 agonist 2‐thiazolylethylamine (0.01–1 mM) did not potentiate the response to impromidine (10 μM). Thus, in contrast to what is generally observed in intact cell preparations from brain, the histamine‐induced cyclic AMP accumulation in astroglial cells is mediated solely by H2 receptors. The small effect shown in neuronal cultures also appears to be mediated by H2 receptors.

Journal

Journal of NeurochemistryWiley

Published: Nov 1, 1990

References

  • Characterization of histamine receptors mediating the stimulation of cyclic AMP accumulation in rabbit cerebral cortical slices
    Al‐Gadi, Al‐Gadi; Hill, Hill
  • The role of calcium in the cyclic AMP response to histamine in rabbit cerebral cortical slices
    Al‐Gadi, Al‐Gadi; Hill, Hill
  • Activation of cyclic AMP‐generating systems in brain membranes and slices by the diterpene forskolin: augmentation of receptor‐mediated responses
    Daly, Daly; Padgett, Padgett; Seamon, Seamon
  • Histamine stimulation of inositol 1‐phosphate accumulation in lithium‐treated slices from regions of guinea pig brain
    Daum, Daum; Downes, Downes; Young, Young
  • Regulation of cyclic AMP accumulation by peptide hormone receptors in immunocytochemically defined astroglial cells
    Evans, Evans; McCarthy, McCarthy; Harden, Harden
  • Histamine increases phospholipid methylation and H 2 ‐receptor‐adenylate cyclase coupling in rat brain
    Ozawa, Ozawa; Segawa, Segawa
  • Corticotropin‐peptide regulation of intracellular cyclic AMP production in cortical neurons in primary culture
    Weiss, Weiss; Sebben, Sebben; Bockaert, Bockaert

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