Colorectal cancer (CRC) is the second and third most
common type of human malignancy in women and men,
respectively, causing 700,000 deaths annually worldwide
. Although the CRC mortality rate has been dramati-
cally decreased owing to signiﬁcant improvement in early
diagnostics, surgical techniques, and chemotherapy, the
disease- free survival rate for patients with advanced- stage
CRC remains low. Because invasion and proliferation are
High expression of DJ- 1 promotes growth and invasion via
the PTEN- AKT pathway and predicts a poor prognosis in
, Qian Chen
, Quan-xing Liu
, Dong Zhou
, Xiao Lu
, Xu-feng Deng
, Hua Yang
& Yuan Qiu
Department of Pathology, The ﬁrst afﬁliated Hospital, Third Military Medical University, Chongqing 400037, China
Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
Department of Cardiothoracic Surgery, First People’s Hospital of Zunyi, Guizhou 563000, China
Department of General Surgery, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, China
© 2018 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use,
distribution and reproduction in any medium, provided the original work is properly cited.
Colorectal cancer, DJ-1, invasion,
Yuan Qiu, Department of General Surgery,
Xinqiao Hospital, the Third Military Medical
University, Chongqing 400037, China.
Hong Zheng, Department of Thoracic
Surgery, Xinqiao Hospital, Third Military
Medical University, Chongqing 400037,
China. Tel: +86-2368-77178;
This work was supported by the National
Natural Science Foundation of China (No.
81500405) and Lijieshou Intestinal Barrier
Academy Foundation for Yuan Qiu.
Received: 10 August 2017; Revised: 12
December 2017; Accepted: 13 December
Cancer Medicine 2018; 7(3):809–819
Cancer cell invasion and unlimited proliferation are key factors in patients with
colorectal cancer (CRC). Increased protein deglycase DJ- 1 in cancer cells is
known to promote tumor growth; however, its role in CRC progression is not
well deﬁned. In this study, we investigated 100 CRC patients with disease stages
I–IV to determine whether DJ- 1 could serve as a prognostic biomarker in CRC.
These results showed that DJ- 1 expression in CRC tissues was higher than that
in normal colon tissues and was associated with the (Tumor Node Metastasis)
TNM stage. CRC patients with low DJ- 1 expression had a longer overall survival
than those with high expression, and multivariate and univariate analyses in-
dicated that DJ- 1 expression was an independent prognostic factor for overall
survival in CRC. Furthermore, DJ- 1 overexpression in two colon cancer cell
lines, HCT116 and SW480, activated protein kinase AKT and downregulated
tumor suppressor PTEN, whereas DJ- 1 knockdown upregulated PTEN expres-
sion and effectively suppressed CRC cell invasion and proliferation both in vitro
and in vivo, revealing a mechanism underlying DJ- 1 pro- oncogenic activity in
CRC. Treatment of MK2206, the speciﬁc AKT inhibitor, signiﬁcantly decreased
DJ- 1- mediated cell proliferation and mobility in vitro. Taken together, these
results suggest that DJ- 1 may be a novel prognostic biomarker and potential
therapeutic target in human CRC.