High‐Affinity Uptake of ( 3 H)Norepinephrine by Primary Astrocyte Cultures and Its Inhibition by Tricyclic Antidepressants

High‐Affinity Uptake of ( 3 H)Norepinephrine by Primary Astrocyte Cultures and Its Inhibition... Abstract: Primary astrocyte cultures from neonatal rat brains show uptake of (3H)norepinephrine ((3H)NE). This uptake has a high‐affinity component with an apparent Km of approximately 3 × 10−7 M. At 10−7 M (3H)NE both the initial rate of uptake and steady‐state content of (3H)NE is inhibited by up to 95% by omission of external Na+. The Na+‐dependent component of this uptake is totally inhibited by the tricyclic antidepressants desipramine (DMI) and amitryptyline with IC50 values of 2 × 10−9 and 4 × 10−8 M, respectively. Inhibition of (3H)NE uptake by DMI shows competitive kinetics. These characteristics are essentially identical to those found for high‐affinity uptake of NE in total membrane or synaptosome fractions from rodent brains and suggests that such uptake in neural tissue is not exclusively neuronal. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

High‐Affinity Uptake of ( 3 H)Norepinephrine by Primary Astrocyte Cultures and Its Inhibition by Tricyclic Antidepressants

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Publisher
Wiley
Copyright
1983 International Society for Neurochemistry
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1983.tb13565.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Primary astrocyte cultures from neonatal rat brains show uptake of (3H)norepinephrine ((3H)NE). This uptake has a high‐affinity component with an apparent Km of approximately 3 × 10−7 M. At 10−7 M (3H)NE both the initial rate of uptake and steady‐state content of (3H)NE is inhibited by up to 95% by omission of external Na+. The Na+‐dependent component of this uptake is totally inhibited by the tricyclic antidepressants desipramine (DMI) and amitryptyline with IC50 values of 2 × 10−9 and 4 × 10−8 M, respectively. Inhibition of (3H)NE uptake by DMI shows competitive kinetics. These characteristics are essentially identical to those found for high‐affinity uptake of NE in total membrane or synaptosome fractions from rodent brains and suggests that such uptake in neural tissue is not exclusively neuronal.

Journal

Journal of NeurochemistryWiley

Published: May 1, 1983

References

  • Immunocytochemical and biochemical analysis of carbonic anhydrase in primary astrocyte cultures from rat brain
    Kimelberg, Kimelberg; Stieg, Stieg; Mazurkiewicz, Mazurkiewicz

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