Growth requirements of immature astrocytes in serum‐free hormonally defined media

Growth requirements of immature astrocytes in serum‐free hormonally defined media A chemically defined serum‐free medium is described that has been optimized for the selective growth of immature astrocytes starting from early postnatal mouse cerebellar cultures. The medium is a modification of one described recently (Fischer et al, 1982) and consists of epidermal growth factor (EGF, 10 nM), transferrin (10 μg/ml), insulin (10 μg/ml), and bovine serum albumin (BSA, 1 mg/ml) in BME with Earle's salts. None of these constituents of the medium alone nor combinations of two of them can stimulate the proliferation and survival of immature astrocytes. Only with a combination of EGF and transferrin together with either insulin or BSA can proliferation of the cells be obtained. For optimal growth all four constituents are needed. However, the immature astrocytes can only grow for a limited time period of about 2–3 wk in this medium. Therefore they can be selected for and characterized but not continously cultivated in this medium. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Research Wiley

Growth requirements of immature astrocytes in serum‐free hormonally defined media

Journal of Neuroscience Research, Volume 12 (4) – Jan 1, 1984

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Publisher
Wiley
Copyright
Copyright © 1984 Alan R. Liss, Inc.
ISSN
0360-4012
eISSN
1097-4547
DOI
10.1002/jnr.490120403
Publisher site
See Article on Publisher Site

Abstract

A chemically defined serum‐free medium is described that has been optimized for the selective growth of immature astrocytes starting from early postnatal mouse cerebellar cultures. The medium is a modification of one described recently (Fischer et al, 1982) and consists of epidermal growth factor (EGF, 10 nM), transferrin (10 μg/ml), insulin (10 μg/ml), and bovine serum albumin (BSA, 1 mg/ml) in BME with Earle's salts. None of these constituents of the medium alone nor combinations of two of them can stimulate the proliferation and survival of immature astrocytes. Only with a combination of EGF and transferrin together with either insulin or BSA can proliferation of the cells be obtained. For optimal growth all four constituents are needed. However, the immature astrocytes can only grow for a limited time period of about 2–3 wk in this medium. Therefore they can be selected for and characterized but not continously cultivated in this medium.

Journal

Journal of Neuroscience ResearchWiley

Published: Jan 1, 1984

References

  • Iron transport and storage proteins
    Aisen, Aisen; Listowsky, Listowsky
  • Epidermal growth factor stimulates DNA‐synthesis of astrocytes in primary cerebellar cultures
    Leutz, Leutz; Schachner, Schachner
  • Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue
    McCarthy, McCarthy; De Vellis, De Vellis
  • Immunocytochemical demonstration of vimentin in astrocytes and ependymal cells of developing and adult mouse nervous system
    Schnitzer, Schnitzer; Franke, Franke; Schachner, Schachner

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