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Growth dynamics of Artemisia annua hairy roots in three culture systems

Growth dynamics of Artemisia annua hairy roots in three culture systems The transient growth of Artemisia annua hairy roots was compared for cultures grown in shake flasks and in bubble column and mist reactors. Instantaneous growth rates were obtained by numerically differentiating the transient biomass measurements. Specific sugar consumption rates showed good agreement with literature values. From the growth rate and sugar consumption rate, the specific yield and maintenance coefficient for sugar were determined for all three culture systems. These values were statistically indistinguishable for roots grown in shake flasks and bubble columns. In contrast, the values for roots grown in bubble columns and mist reactors were statistically different, suggesting that sugar utilization by roots grown in these two systems may be different. By measuring respiration rates in the bubble column reactor we also determined the actual biomass yield and maintenance coefficient for O2 and CO2. Together with an elemental analysis of the roots, this allowed us to obtain a reasonable carbon balance. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 428–443, 2003. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology and Bioengineering Wiley

Growth dynamics of Artemisia annua hairy roots in three culture systems

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References (46)

Publisher
Wiley
Copyright
Copyright © 2003 Wiley Periodicals, Inc., A Wiley Company
ISSN
0006-3592
eISSN
1097-0290
DOI
10.1002/bit.10685
pmid
12800137
Publisher site
See Article on Publisher Site

Abstract

The transient growth of Artemisia annua hairy roots was compared for cultures grown in shake flasks and in bubble column and mist reactors. Instantaneous growth rates were obtained by numerically differentiating the transient biomass measurements. Specific sugar consumption rates showed good agreement with literature values. From the growth rate and sugar consumption rate, the specific yield and maintenance coefficient for sugar were determined for all three culture systems. These values were statistically indistinguishable for roots grown in shake flasks and bubble columns. In contrast, the values for roots grown in bubble columns and mist reactors were statistically different, suggesting that sugar utilization by roots grown in these two systems may be different. By measuring respiration rates in the bubble column reactor we also determined the actual biomass yield and maintenance coefficient for O2 and CO2. Together with an elemental analysis of the roots, this allowed us to obtain a reasonable carbon balance. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 428–443, 2003.

Journal

Biotechnology and BioengineeringWiley

Published: Aug 20, 2003

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