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D. Ramakrishnan, W. Curtis (1994)
Fluid Dynamic Studies on Plant Root Cultures for Application to Bioreactor Design
J. Bordonaro, W. Curtis (2000)
Inhibitory role of root hairs on transport within root culture bioreactors.Biotechnology and bioengineering, 70 2
D. Rho, G. André (1991)
Growth and stoichiometry of a Catharanthus roseus cell suspension culture grown under nitrogen‐limiting conditionsBiotechnology and Bioengineering, 38
Ramakrishnan, Luyk, Curtis (1999)
Monitoring biomass in root culture systems.Biotechnology and bioengineering, 62 6
Kian Kwok, P. Doran (1995)
Kinetic and Stoichiometric Analysis of Hairy Roots in a Segmented Bubble Column ReactorBiotechnology Progress, 11
Yoo-Jeong Kim, P. Weathers, B. Wyslouzil (2002)
Growth of Artemisia annua hairy roots in liquid‐ and gas‐phase reactorsBiotechnology and Bioengineering, 80
P. Bevington, D. Robinson, G. Bunce (1993)
Data Reduction and Error Analysis for the Physical Sciences, 2nd ed.American Journal of Physics, 61
G. Williams, P. Doran (1999)
Investigation of liquid-solid hydrodynamic boundary layers and oxygen requirements in hairy root cultures.Biotechnology and bioengineering, 64 6
(cid:2) X/CO 2 actual biomass yield from carbon dioxide [(g biomass) (cid:1) (mmol CO 2 ) −1 ]
T. Shiao, P. Doran (2000)
Root hairiness: effect on fluid flow and oxygen transfer in hairy root cultures.Journal of biotechnology, 83 3
Kanokwan Kanokwaree, P. Doran (1997)
Effect of inoculum size on growth of Atropa belladonna hairy roots in shake flasksJournal of Fermentation and Bioengineering, 84
Y. Kim, B. Wyslouzil, P. Weathers (2001)
A comparative study of mist and bubble column reactors in the in vitro production of artemisininPlant Cell Reports, 20
W. Gulik, H. Hoopen, J. Heijnen (1992)
Kinetics and stoichiometry of growth of plant cell cultures of Catharanthus roseus and Nicotiana tabacum in batch and continuous fermentorsBiotechnology and Bioengineering, 40
G. Jung, D. Tepfer (1987)
Use of genetic transformation by the Ri T-DNA of Agrobacterium rhizogenes to stimulate biomass and tropane alkaloid production in Atropa belladonna and Calystegia sepium roots grown in vitroPlant Science, 50
R. Bhadra, J. Shanks (1997)
Transient studies of nutrient uptake, growth, and indole alkaloid accumulation in heterotrophic cultures of hairy roots of Catharanthus roseus.Biotechnology and bioengineering, 55 3
J. Riu, F. Rius (1996)
Assessing the accuracy of analytical methods using linear regression with errors in both axes.Analytical chemistry, 68 11
T. Breen, J. Zar (1986)
Biostatistical Analysis (2nd ed.).Journal of the American Statistical Association, 81
Y (cid:2) P/S actual byproduct yield from substrate [g (cid:1) (g glucose equivalent) −1 ]
J. Sharp, P. Doran (1990)
Characteristics of growth and tropane alkaloid synthesis in Atropa belladonna roots transformed by Agrobacterium rhizogenesJournal of Biotechnology, 16
L. Toivonen, M. Ojala, V. Kauppinen (1990)
Indole alkaloid production by hairy root cultures ofCatharanthus roseus: Growth kinetics and fermentationBiotechnology Letters, 12
D. Ryu, S. Lee, R. Romani (1990)
Determination of growth rate for plant cell cultures: comparative studies.Biotechnology and bioengineering, 35 3
sugar consumption rate associated with growth
W. Press, S. Teukolsky (1990)
Numerical recipesComputers in Physics, 4
S. McKelvey, J. Gehrig, K. Hollar, W. Curtis (1993)
Growth of Plant Root Cultures in Liquid‐ and Gas‐Dispersed Reactor EnvironmentsBiotechnology Progress, 9
A. Kato, S. Nagai (1979)
Energetics of tobacco cells, Nicotiana tabacum L., growing on sucrose mediumEuropean journal of applied microbiology and biotechnology, 7
L. Tescione, D. Ramakrishnan, W. Curtis (1997)
The role of liquid mixing and gas-phase dispersion in a submerged, sparged root reactor.Enzyme and microbial technology, 20 3
Kanokwan Kanokwaree, P. Doran (1998)
Application of Membrane Tubing Aeration and Perfluorocarbon To Improve Oxygen Delivery to Hairy Root CulturesBiotechnology Progress, 14
(cid:2) actual biomass yield from oxygen [(g biomass) (cid:1) (mmol O ) −1 ] and now x ( t ) is the same biomass concentration (g/L) as in
M. Kino‐oka, S. Tsutsumi, S. Tone (1996)
OXYGEN TRANSFER IN BIOREACTOR FOR CULTURE OF PLANT HAIRY ROOTSJournal of Chemical Engineering of Japan, 29
J. Bailey, D. Ollis (1976)
Biochemical Engineering Fundamentals
K. Jung, S. Kwak, Jang-R. Liu (1998)
Procedure for biomass estimation considering the change in biomass volume during high density culture of hairy rootsJournal of Fermentation and Bioengineering, 85
m CO 2 maintenance coefficient for carbon dioxide [(mmol CO 2 ) (cid:1) (g biomass) −1 day −1 ]
(1994)
Studies in plant science. 4. Advances in plant biotechnology: production of secondary metabolites
M. Taya, M. Hegglin, J. Přenosil, J. Bourne (1989)
On-line monitoring of cell growth in plant tissue cultures by conductometryEnzyme and Microbial Technology, 11
S. Schnapp, W. Curtis, R. Bressan, P. Hasegawa (1991)
Growth Yields and Maintenance Coefficients of Unadapted and NaCl-Adapted Tobacco Cells Grown in Semicontinuous Culture.Plant physiology, 96 4
S. Pirt (1965)
The maintenance energy of bacteria in growing culturesProceedings of the Royal Society of London. Series B. Biological Sciences, 163
(1999)
Biostatistical analysis, 4th ed. Upper Saddle River, NJ: Prentice Hall
P. Weathers, R. Cheetham, E. Follansbee, K. Teoh (1994)
Artemisinin production by transformed roots of Artemisia annuaBiotechnology Letters, 16
biomass) (cid:1) (g glucose equivalent) −1 ]
Change in the DW/FW ratio as a function of the dry weight biomass concentration in shake flasks and the reactors. The error bars indicate standard deviations
O. Gamborg, R. Miller, K. Ojima (1968)
Nutrient requirements of suspension cultures of soybean root cells.Experimental cell research, 50 1
(2001)
Assessment of bioreactors for transformed root cultures
Gary Williams, P. Doran (2000)
Hairy Root Culture in a Liquid‐Dispersed Bioreactor: Characterization of Spatial HeterogeneityBiotechnology Progress, 16
R. Taticek, M. Moo-young, R. Legge (1990)
Effect of bioreactor configuration on substrate uptake by cell suspension cultures of the plant Eschscholtzia californicaApplied Microbiology and Biotechnology, 35
M. Taya, A. Yoyama, O. Kondo, Takeshi Kobayashi, C. Matsui (1989)
Growth Characteristics of Plant Hairy Roots and Their Cultures in BioreactorsJournal of Chemical Engineering of Japan, 22
W. Press, B. Flannery, S. Teukolsky, W. Vetterling (1992)
Numerical Recipes in Fortran 77: The Art of Scientific Computing 2nd Editionn - Volume 1 of Fortran Numerical Recipes
The transient growth of Artemisia annua hairy roots was compared for cultures grown in shake flasks and in bubble column and mist reactors. Instantaneous growth rates were obtained by numerically differentiating the transient biomass measurements. Specific sugar consumption rates showed good agreement with literature values. From the growth rate and sugar consumption rate, the specific yield and maintenance coefficient for sugar were determined for all three culture systems. These values were statistically indistinguishable for roots grown in shake flasks and bubble columns. In contrast, the values for roots grown in bubble columns and mist reactors were statistically different, suggesting that sugar utilization by roots grown in these two systems may be different. By measuring respiration rates in the bubble column reactor we also determined the actual biomass yield and maintenance coefficient for O2 and CO2. Together with an elemental analysis of the roots, this allowed us to obtain a reasonable carbon balance. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 83: 428–443, 2003.
Biotechnology and Bioengineering – Wiley
Published: Aug 20, 2003
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