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S. Chun, H. Billig, J. Tilly, I. Furuta, A. Tsafriri, A. Hsueh (1994)
Gonadotropin suppression of apoptosis in cultured preovulatory follicles: mediatory role of endogenous insulin-like growth factor I.Endocrinology, 135 5
H. Fraser, T. Bramley, W. Miller, R. Sharpe (1986)
Extra pituitary actions of LHRH analogues in tissues of the human female and investigation of the existence and function of LHRH-like peptides.Progress in clinical and biological research, 225
A., L., Johnson, J., T., Bridgham, P. Witty, Tilly (1996)
Susceptibility of avian ovarian granulosa cells to apoptosis is dependent upon stage of follicle development and is related to endogenous levels of bcl-xlong gene expression.Endocrinology, 137 5
R. Clayton, J. Harwood, K. Catt (1979)
Gonadotropin-releasing hormone analogue binds to luteal cells and inhibits progesterone productionNature, 282
R. Nahum, Y. Beyth, S. Chun, A. Hsueh, A. Tsafriri (1996)
Early onset of deoxyribonucleic acid fragmentation during atresia of preovulatory ovarian follicles in rats.Biology of reproduction, 55 5
J. Tilly, K. Tilly, Maura Kenton, A. Johnson (1995)
Expression of members of the bcl-2 gene family in the immature rat ovary: equine chorionic gonadotropin-mediated inhibition of granulosa cell apoptosis is associated with decreased bax and constitutive bcl-2 and bcl-xlong messenger ribonucleic acid levels.Endocrinology, 136 1
Chun Peng, N. Fan, M. Ligier, J. Väänänen, Peter Leung (1994)
Expression and regulation of gonadotropin-releasing hormone (GnRH) and GnRH receptor messenger ribonucleic acids in human granulosa-luteal cells.Endocrinology, 135 5
J Olofsson, C Conti, P. Leung (1995)
Homologous and heterologous regulation of gonadotropin-releasing hormone receptor gene expression in preovulatory rat granulosa cells.Endocrinology, 136 3
P. Jones, A. Hsueh (1981)
Direct effects of gonadotropin releasing hormone and its antagonist upon ovarian functions stimulated by FSH, prolactin, ans LH.Biology of reproduction, 24 4
L. Boise, M. González‐García, C. Postema, Liyun Ding, T. Lindsten, L. Turka, X. Mao, G. Núñez, C. Thompson (1993)
bcl-x, a bcl-2-related gene that functions as a dominant regulator of apoptotic cell deathCell, 74
E. Mcgee, N. Spears, S. Minami, S. Hsu, S. Chun, H. Billig, A. Hsueh (1997)
Preantral Ovarian Follicles in Serum-Free Culture: Suppression of Apoptosis after Activation of the Cyclic Guanosine 3',5'-Monophosphate Pathway and Stimulation of Growth and Differentiation by Follicle-Stimulating Hormone.Endocrinology, 138 6
P. Novick, P. Brennwald (1993)
Friends and family: The role of the rab GTPases in vesicular trafficCell, 75
A. Zeleznik, L. Ihrig, S. Bassett (1989)
Developmental expression of Ca++/Mg++-dependent endonuclease activity in rat granulosa and luteal cells.Endocrinology, 125 4
J. Tilly, H. Billig, K. Kowalski, A. Hsueh (1992)
Epidermal growth factor and basic fibroblast growth factor suppress the spontaneous onset of apoptosis in cultured rat ovarian granulosa cells and follicles by a tyrosine kinase-dependent mechanism.Molecular endocrinology, 6 11
S. Goubau, Chris Bond, John Adelman, Vikram Misra, Michael Hynes, Gilbert Schultz, Bruce Murphy (1992)
Partial characterization of the gonadotropin-releasing hormone (GnRH) gene transcript in the rat ovary.Endocrinology, 130 5
E. Mcgee, A. Hsueh (2000)
Initial and cyclic recruitment of ovarian follicles.Endocrine reviews, 21 2
R. Srivastava, R. Sridaran (1994)
Inhibition of luteal steroidogenesis by two LHRH antagonists (Nal-Glu and Nal-Arg antagonists) in the pregnant rat.Endocrine research, 20 4
M. Knecht, A. Amsterdam, K. Catt (1982)
Inhibition of granulosa cell differentiation by gonadotropin-releasing hormone.Endocrinology, 110 3
S. Chun, K. Eisenhauer, S. Minami, H. Billig, E. Perlas, A. Hsueh (1996)
Hormonal regulation of apoptosis in early antral follicles: follicle-stimulating hormone as a major survival factor.Endocrinology, 137 4
P. Leung, G. Steele (1992)
Intracellular signaling in the gonads.Endocrine reviews, 13 3
C. Séguin, G. Pelletier, D. Dubé, F. Labrie (1982)
Distribution of luteinizing hormone-releasing hormone receptors in the rat ovaryRegulatory Peptides, 4
Anne Hirshfield, A. Midgley (1978)
Morphometric analysis of follicular development in the rat.Biology of reproduction, 19 3
Demetrios Minaretzis, M. Jakubowski, J. Mortola, S. Pavlou (1995)
Gonadotropin-releasing hormone receptor gene expression in human ovary and granulosa-lutein cells.The Journal of clinical endocrinology and metabolism, 80 2
J. Tilly, Kim Kowalski, A. Johnson, A. Hsueh (1991)
Involvement of apoptosis in ovarian follicular atresia and postovulatory regression.Endocrinology, 129 5
A. Hsueh, E. Adashi, Phillip Jones, T. Welsh (1984)
Hormonal regulation of the differentiation of cultured ovarian granulosa cells.Endocrine reviews, 5 1
P. Whitelaw, K. Eidne, R. Sellar, C. Smyth, S. Hillier (1995)
Gonadotropin-releasing hormone receptor messenger ribonucleic acid expression in rat ovary.Endocrinology, 136 1
E. Mcgee, S. Chun, Susan Lai, You-e He, A. Hsueh (1999)
Keratinocyte growth factor promotes the survival, growth, and differentiation of preantral ovarian follicles.Fertility and sterility, 71 4
A. Palumbo, J. Yeh (1994)
In situ localization of apoptosis in the rat ovary during follicular atresia.Biology of reproduction, 51 5
Jonathan Tilly, K. Tilly (1995)
Inhibitors of oxidative stress mimic the ability of follicle-stimulating hormone to suppress apoptosis in cultured rat ovarian follicles.Endocrinology, 136 1
C. Andreu, F. Parborell, S. Vanzulli, H. Chemes, M. Tesone (1998)
Regulation of follicular luteinization by a gonadotropin‐releasing hormone agonist: Relationship between steroidogenesis and apoptosisMolecular Reproduction and Development, 51
A. Gougeon (1996)
Regulation of ovarian follicular development in primates: facts and hypotheses.Endocrine reviews, 17 2
P. Conn, A. Hsueh, W. Crowley (1984)
Gonadotropin-releasing hormone: molecular and cell biology, physiology, and clinical applications.Federation proceedings, 43 9
S. Stojilkovic, J. Reinhart, K. Catt (1994)
Gonadotropin-releasing hormone receptors: structure and signal transduction pathways.Endocrine reviews, 15 4
J. Tilly, K. Kowalski, D. Schomberg, A. Hsueh (1992)
Apoptosis in atretic ovarian follicles is associated with selective decreases in messenger ribonucleic acid transcripts for gonadotropin receptors and cytochrome P450 aromatase.Endocrinology, 131 4
H. Billig, I. Furuta, A. Hsueh (1994)
Gonadotropin-releasing hormone directly induces apoptotic cell death in the rat ovary: biochemical and in situ detection of deoxyribonucleic acid fragmentation in granulosa cells.Endocrinology, 134 1
H. Guerrero, P. Stein, R. Asch, E. Fried, M. Tesone (1993)
Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells.Fertility and sterility, 59 4
(1998)
Cell death and species propagation: molecular and genetic aspects of apoptosis in the vertebrate female gonad
G. Erickson, D. Li, R. Sadrkhanloo, X. Liu, S. Shimasaki, N. Ling (1994)
Extrapituitary actions of gonadotropin-releasing hormone: stimulation of insulin-like growth factor-binding protein-4 and atresia.Endocrinology, 134 3
D. Pieper, J. Richards, J. Marshall (1981)
Ovarian gonadotropin-releasing hormone (GnRH) receptors: characterization, distribution, and induction by GnRH.Endocrinology, 108 4
L. Birnbaumer, N. Shahabi, J. Rivier, W. Vale (1985)
Evidence for a physiological role of gonadotropin-releasing hormone (GnRH) or GnRH-like material in the ovary.Endocrinology, 116 4
V. Papadopoulos, A. Dharmarajan, H. Li, M. Culty, M. Lemay, R. Sridaran (1999)
Mitochondrial peripheral-type benzodiazepine receptor expression. Correlation with gonadotropin-releasing hormone (GnRH) agonist-induced apoptosis in the corpus luteum.Biochemical pharmacology, 58 9
A. Hirshfield (1991)
Development of follicles in the mammalian ovary.International review of cytology, 124
E. White (1996)
Life, death, and the pursuit of apoptosis.Genes & development, 10 1
Apoptosis is the biological process by which follicular cells are eliminated in atretic follicles. The aim of the present study was to examine the in vitro effect of a GnRH‐a (leuprolide acetate, LA) and its interactions with FSH, dibutyryl cAMP, and growth factors (IGF‐I, EGF, and FGF) on follicular apoptosis in early antral ovarian follicles obtained from prepubertal DES‐ treated rats. Follicles cultured 24 hr in the absence of hormones showed spontaneous onset of apoptotic DNA fragmentation. The presence of FSH suppressed the spontaneous onset of apoptotic DNA fragmentation (75–85%). Quantitative estimation of DNA cleavage from ovarian follicles revealed no significant changes in DNA fragmentation after in vitro LA treatment (1–100 ng/ml). However, coincubation with LA interfered partially with the effects of FSH on apoptosis suppression. This apoptosis suppression was also obtained by treatment with dibutyryl cAMP (80%), and was partially prevented by the presence of LA in the cultures. Follicles were cultured 24 hr with FGF, EGF, or IGF‐I, and these factors suppressed DNA fragmentation (70, 60, and 70% respectively), while the presence of LA (100 ng/ml) in the culture medium prevented this effect. In conclusion, we show that the rescue from apoptotic DNA fragmentation produced in early antral follicles by FSH, cAMP, and growth factors, is prevented by coincubation with LA. This GnRH analog would thus interfere in the pathway of FSH, cAMP and/or growth factors by an as yet unknown mechanism. Mol. Reprod. Dev. 60: 241–247, 2001. © 2001 Wiley‐Liss, Inc.
Molecular Reproduction & Development – Wiley
Published: Oct 1, 2001
Keywords: ovary; GnRH; apoptosis; growth factors; early antral follicles
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