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Glucose uptake and ornithine decarboxylase activity in tetradecanoyl phorbol acetate non‐proliferative variants

Glucose uptake and ornithine decarboxylase activity in tetradecanoyl phorbol acetate... 10.1002/jcp.1041140310.abs The potent tumor promoter 12‐0‐tetradecanoyl phorbol‐13‐acetate (TPA) is alos an excellent mitogen for 3T3 cells. We have previously isolated two independent variants, 3T3‐TNR‐2 and 3T3‐TNR‐9, that are unable to divide in response to TPA (Butler‐Gralla and Herschman, 1981). We have now tested tow components of the pleiotypic response, elevation of 2‐deoxyglucose uptake and ornithine decarboxylase induction, in these cells. Basal levels of 2‐deoxyglucose uptake were nearly tenfold higher in confluent 3T3‐TNR‐2 and 3T3‐TNR‐9 cells than in 3T3 cells. In contrast, basal ornithine decarboxylase levels were five‐ to tenfold lower in the variants. TPA stimulation of 2‐deoxyglucose uptake was as great in absolute terms in the variant cell lines as that of 3T3 cells but was only half that observed with serum. TPA was unable to induce any elevation of ornithine decarboxylase in 3T3‐TNR‐9 cells. treated with TPA, the maximal specific activity in the variant was less than the unstimulated value for 3T3 cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Cellular Physiology Wiley

Glucose uptake and ornithine decarboxylase activity in tetradecanoyl phorbol acetate non‐proliferative variants

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References (26)

Publisher
Wiley
Copyright
Copyright © 1983 Wiley‐Liss, Inc.
ISSN
0021-9541
eISSN
1097-4652
DOI
10.1002/jcp.1041140310
pmid
6833404
Publisher site
See Article on Publisher Site

Abstract

10.1002/jcp.1041140310.abs The potent tumor promoter 12‐0‐tetradecanoyl phorbol‐13‐acetate (TPA) is alos an excellent mitogen for 3T3 cells. We have previously isolated two independent variants, 3T3‐TNR‐2 and 3T3‐TNR‐9, that are unable to divide in response to TPA (Butler‐Gralla and Herschman, 1981). We have now tested tow components of the pleiotypic response, elevation of 2‐deoxyglucose uptake and ornithine decarboxylase induction, in these cells. Basal levels of 2‐deoxyglucose uptake were nearly tenfold higher in confluent 3T3‐TNR‐2 and 3T3‐TNR‐9 cells than in 3T3 cells. In contrast, basal ornithine decarboxylase levels were five‐ to tenfold lower in the variants. TPA stimulation of 2‐deoxyglucose uptake was as great in absolute terms in the variant cell lines as that of 3T3 cells but was only half that observed with serum. TPA was unable to induce any elevation of ornithine decarboxylase in 3T3‐TNR‐9 cells. treated with TPA, the maximal specific activity in the variant was less than the unstimulated value for 3T3 cells.

Journal

Journal of Cellular PhysiologyWiley

Published: Mar 1, 1983

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