Genetic differentiation between the clam species Ruditapes decussatus (grooved carpet shell) and Venerupis pullastra (pullet carpet shell) by PCR–SSCP analysis

Genetic differentiation between the clam species Ruditapes decussatus (grooved carpet shell) and... Genetic differentiation of the clam species Ruditapes decussatus (grooved carpet shell) and Venerupis pullastra (pullet carpet shell) has been achieved based on polymerase chain reaction–single‐strand conformation polymorphism (PCR–SSCP) analysis. A short fragment (150 bp) of the α‐actin gene was amplified by PCR. Amplicons were denatured to obtain single‐stranded DNA, electrophoresed on a non‐denaturing polyacrylamide gel and visualised by silver staining for detection of SSCPs. Species‐specific DNA band patterns were obtained for R decussatus and V pullastra, allowing clear differentiation of the two clam species. © 2002 Society of Chemical Industry http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of the Science of Food and Agriculture Wiley

Genetic differentiation between the clam species Ruditapes decussatus (grooved carpet shell) and Venerupis pullastra (pullet carpet shell) by PCR–SSCP analysis

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Publisher
Wiley
Copyright
© 2002 Society of Chemical Industry
ISSN
0022-5142
eISSN
1097-0010
D.O.I.
10.1002/jsfa.1117
Publisher site
See Article on Publisher Site

Abstract

Genetic differentiation of the clam species Ruditapes decussatus (grooved carpet shell) and Venerupis pullastra (pullet carpet shell) has been achieved based on polymerase chain reaction–single‐strand conformation polymorphism (PCR–SSCP) analysis. A short fragment (150 bp) of the α‐actin gene was amplified by PCR. Amplicons were denatured to obtain single‐stranded DNA, electrophoresed on a non‐denaturing polyacrylamide gel and visualised by silver staining for detection of SSCPs. Species‐specific DNA band patterns were obtained for R decussatus and V pullastra, allowing clear differentiation of the two clam species. © 2002 Society of Chemical Industry

Journal

Journal of the Science of Food and AgricultureWiley

Published: Jun 1, 2002

References

  • Identification of fish species using random amplified polymorphic DNA (RAPD)
    Partis, Partis; Wells, Wells
  • Application of PCR–SSCP to species identification of fishery products
    Rehbein, Rehbein; Kress, Kress; Schmidt, Schmidt
  • Screening techniques for detecting allelic variation in DNA sequences
    Lessa, Lessa; Applebaum, Applebaum
  • DNA‐based methods for food authentication
    Lockley, Lockley; Bardsley, Bardsley
  • How sensitive is PCR–SSCP?
    Hayashi, Hayashi; Yandell, Yandell

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