GABAergic Interneurons are the Major Postsynaptic Targets of Median Raphe Afferents in the Rat Dentate Gyrus

GABAergic Interneurons are the Major Postsynaptic Targets of Median Raphe Afferents in the Rat... The termination pattern of median raphe axons was studied in the rat dentate gyrus using Phaseolus vulgaris leucoagglutinin as an anterograde tracer, in combination with postembedding immunostaining for γ‐amino‐butyric acid (GABA), and pre‐embedding immunostaining for calbindin D28k, parvalbumin and GABA. Postembedding immunogold staining for GABA revealed that the majority (73.7%) of anterogradely labelled median raphe boutons make synaptic contacts with GABA‐immunoreactive postsynaptic targets, mainly with dendritic shafts and perikarya. Pre‐embedding immunocytochemical double staining for the anterograde tracer and GABA confirmed the electron microscopic results and showed that varicose median raphe axons establish multiple contacts with fusiform interneurons in the hilus and different types of basket cells in the granule cell layer. Some of the innervated cells were shown to contain calbindin D28k, whereas GABAergic interneurons containing another calcium‐binding protein, parvalbumin, were never seen to receive multiple contacts from axons of raphe origin. Our results suggest that serotonergic median raphe fibres influence the firing of dentate granule cells via local inhibitory interneurons. The mechanism of using these interneurons with extensive local connections as monosynaptic targets may explain the great efficacy of this pathway in the control of hippocampal electrical activity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Neuroscience Wiley

GABAergic Interneurons are the Major Postsynaptic Targets of Median Raphe Afferents in the Rat Dentate Gyrus

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Publisher
Wiley
Copyright
Copyright © 1992 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0953-816X
eISSN
1460-9568
DOI
10.1111/j.1460-9568.1992.tb00861.x
Publisher site
See Article on Publisher Site

Abstract

The termination pattern of median raphe axons was studied in the rat dentate gyrus using Phaseolus vulgaris leucoagglutinin as an anterograde tracer, in combination with postembedding immunostaining for γ‐amino‐butyric acid (GABA), and pre‐embedding immunostaining for calbindin D28k, parvalbumin and GABA. Postembedding immunogold staining for GABA revealed that the majority (73.7%) of anterogradely labelled median raphe boutons make synaptic contacts with GABA‐immunoreactive postsynaptic targets, mainly with dendritic shafts and perikarya. Pre‐embedding immunocytochemical double staining for the anterograde tracer and GABA confirmed the electron microscopic results and showed that varicose median raphe axons establish multiple contacts with fusiform interneurons in the hilus and different types of basket cells in the granule cell layer. Some of the innervated cells were shown to contain calbindin D28k, whereas GABAergic interneurons containing another calcium‐binding protein, parvalbumin, were never seen to receive multiple contacts from axons of raphe origin. Our results suggest that serotonergic median raphe fibres influence the firing of dentate granule cells via local inhibitory interneurons. The mechanism of using these interneurons with extensive local connections as monosynaptic targets may explain the great efficacy of this pathway in the control of hippocampal electrical activity.

Journal

European Journal of NeuroscienceWiley

Published: Feb 1, 1992

References

  • Pharmacologically distinct actions of serotonin on single pyramidal neurons of the rat hippocampus recorded in vitro
    Andrade, Andrade; Nicoll, Nicoll
  • Reduction of long‐term potentiation in the dentate gyrus of the rat following selective depletion of monoamines
    Bliss, Bliss; Goddard, Goddard; Riives, Riives
  • 5‐hydroxytryptamine receptor subtypes
    Peroutka, Peroutka
  • Effects of serotonin releasers on dentate granule cell excitability in the rat
    Richter‐Levin, Richter‐Levin; Segal, Segal
  • The action of serotonin in the rat hippocampal slice preparation
    Segal, Segal
  • GABAergic cells in the dentate gyrus appear to be local circuit and projection neurons
    Seress, Seress; Ribak, Ribak
  • Raphe influences on neuronal transmission from perforant pathway through dentate gyrus
    Winson, Winson

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