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Fully glycerol‐independent microbial production of 1, 3‐propanediol via non‐natural pathway: Paving the way to success with synthetic tiles

Fully glycerol‐independent microbial production of 1, 3‐propanediol via non‐natural pathway:... See accompanying article by Zhen Chen et al. DOI: 10.1002/biot.201400235 A number of inventive genetic and metabolic engineering strategies towards improved 1,3‐propanediol (1,3‐PDO) production have been reported to date. So far, all of the strategies relied on bioconversion of glycerol. Either glycerol has been used as a primary substrate in the culture medium, or glycerol was an intermediate in a synthetic pathway leading from glucose to 1,3‐PDO. The article by Chen et al. [ 1 ] in this issue of Biotechnology Journal constitutes a paradigm shift in our understanding of microbial 1,3‐PDO production. The authors constructed a fully glycerol‐independent pathway of 1,3‐PDO synthesis from glucose by recruiting native metabolites of central carbon and nitrogen metabolism. The connection between the native and heterologous parts was secured by an engineered protein with modified characteristics, which constitutes a substantial added value of this contribution. The paper illustrates an excellent example of a successful synthetic biology approach with application in industrial biotechnology. Glycerol is the conventional substrate for 1,3‐PDO production, nevertheless, efforts towards development of alternative, non‐natural routes of 1,3‐PDO synthesis have been pursued. In general, three different strategies can be adopted: i) engineering natural 1,3‐PDO producers, to endow them with the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Journal Wiley

Fully glycerol‐independent microbial production of 1, 3‐propanediol via non‐natural pathway: Paving the way to success with synthetic tiles

Biotechnology Journal , Volume 10 (2) – Feb 1, 2015

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References (8)

Publisher
Wiley
Copyright
Copyright © 2015 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1860-6768
eISSN
1860-7314
DOI
10.1002/biot.201400360
pmid
25371377
Publisher site
See Article on Publisher Site

Abstract

See accompanying article by Zhen Chen et al. DOI: 10.1002/biot.201400235 A number of inventive genetic and metabolic engineering strategies towards improved 1,3‐propanediol (1,3‐PDO) production have been reported to date. So far, all of the strategies relied on bioconversion of glycerol. Either glycerol has been used as a primary substrate in the culture medium, or glycerol was an intermediate in a synthetic pathway leading from glucose to 1,3‐PDO. The article by Chen et al. [ 1 ] in this issue of Biotechnology Journal constitutes a paradigm shift in our understanding of microbial 1,3‐PDO production. The authors constructed a fully glycerol‐independent pathway of 1,3‐PDO synthesis from glucose by recruiting native metabolites of central carbon and nitrogen metabolism. The connection between the native and heterologous parts was secured by an engineered protein with modified characteristics, which constitutes a substantial added value of this contribution. The paper illustrates an excellent example of a successful synthetic biology approach with application in industrial biotechnology. Glycerol is the conventional substrate for 1,3‐PDO production, nevertheless, efforts towards development of alternative, non‐natural routes of 1,3‐PDO synthesis have been pursued. In general, three different strategies can be adopted: i) engineering natural 1,3‐PDO producers, to endow them with the

Journal

Biotechnology JournalWiley

Published: Feb 1, 2015

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