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Zheng Ma, Z. Rao, Liyuan Xu, Xian-Yan Liao, H. Fang, B. Zhuge, J. Zhuge (2010)
Expression of dha Operon Required for 1,3-PD Formation in Escherichia coli and Saccharomyces cerevisiaeCurrent Microbiology, 60
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(2010)
pression of dha operon required for 1 , 3 - PD formation in Escherichia coli and Saccharomyces cerevisiae
See accompanying article by Zhen Chen et al. DOI: 10.1002/biot.201400235 A number of inventive genetic and metabolic engineering strategies towards improved 1,3‐propanediol (1,3‐PDO) production have been reported to date. So far, all of the strategies relied on bioconversion of glycerol. Either glycerol has been used as a primary substrate in the culture medium, or glycerol was an intermediate in a synthetic pathway leading from glucose to 1,3‐PDO. The article by Chen et al. [ 1 ] in this issue of Biotechnology Journal constitutes a paradigm shift in our understanding of microbial 1,3‐PDO production. The authors constructed a fully glycerol‐independent pathway of 1,3‐PDO synthesis from glucose by recruiting native metabolites of central carbon and nitrogen metabolism. The connection between the native and heterologous parts was secured by an engineered protein with modified characteristics, which constitutes a substantial added value of this contribution. The paper illustrates an excellent example of a successful synthetic biology approach with application in industrial biotechnology. Glycerol is the conventional substrate for 1,3‐PDO production, nevertheless, efforts towards development of alternative, non‐natural routes of 1,3‐PDO synthesis have been pursued. In general, three different strategies can be adopted: i) engineering natural 1,3‐PDO producers, to endow them with the
Biotechnology Journal – Wiley
Published: Feb 1, 2015
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