Frequent infection with a viral pathogen, parvovirus B19, in rheumatic diseases of childhood

Frequent infection with a viral pathogen, parvovirus B19, in rheumatic diseases of childhood Objective To find further evidence of the association of parvovirus B19 infection with juvenile rheumatic diseases, and to get new insights into the immunopathogenesis of these diseases. Methods Paired serum and synovial fluid samples from 74 children with rheumatic disease were analyzed with respect to their content of viral DNA and antibodies directed against the B19 viral proteins VP1, VP2, and NS1. Control sera from 124 children with noninflammatory bone diseases or growth retardation were also analyzed. The sequence of the viral DNA, amplified by polymerase chain reaction (PCR), was determined. IgG‐complexed virus was isolated from sera and synovial fluid by adsorption to protein A beads. The amount of free virus versus immunocomplexed virus particles was determined by quantification of the viral genomes by quantitative PCR. Results Twenty‐six of the 74 patients (35%) had detectable amounts of parvovirus B19 DNA in the serum (n = 22 (30%)) and/or the synovial fluid (n = 16 (22%)), whereas only 9 of the 124 control sera (7%) were positive for the viral DNA (P < 0.0001). Forty‐six patients (62%) had serum IgG against the structural proteins, indicating past infection with B19. NS1‐specific antibodies were detected in sera from 29 patients (39%) and 27 controls (22%) (P < 0.001). In addition, 3 patients (4%) showed VP2‐specific IgM. In 15 patients, viral DNA could be repeatedly detected in followup samples of serum and synovial fluid. Sequencing revealed low‐degree nucleotide variations that are in the range of genotype 1 of parvovirus B19. Immunocomplexed virus was present in varying amounts, both in the sera and in the synovial fluid samples. Conclusion Parvovirus B19 is frequently found in serum or synovial fluid of children with rheumatism. The rate of persistent B19 infection in these patients is significantly higher than in age‐matched controls. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arthritis & Rheumatology Wiley

Frequent infection with a viral pathogen, parvovirus B19, in rheumatic diseases of childhood

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Publisher
Wiley
Copyright
Copyright © 2003 by the American College of Rheumatology
ISSN
0004-3591
eISSN
1529-0131
DOI
10.1002/art.10979
Publisher site
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Abstract

Objective To find further evidence of the association of parvovirus B19 infection with juvenile rheumatic diseases, and to get new insights into the immunopathogenesis of these diseases. Methods Paired serum and synovial fluid samples from 74 children with rheumatic disease were analyzed with respect to their content of viral DNA and antibodies directed against the B19 viral proteins VP1, VP2, and NS1. Control sera from 124 children with noninflammatory bone diseases or growth retardation were also analyzed. The sequence of the viral DNA, amplified by polymerase chain reaction (PCR), was determined. IgG‐complexed virus was isolated from sera and synovial fluid by adsorption to protein A beads. The amount of free virus versus immunocomplexed virus particles was determined by quantification of the viral genomes by quantitative PCR. Results Twenty‐six of the 74 patients (35%) had detectable amounts of parvovirus B19 DNA in the serum (n = 22 (30%)) and/or the synovial fluid (n = 16 (22%)), whereas only 9 of the 124 control sera (7%) were positive for the viral DNA (P < 0.0001). Forty‐six patients (62%) had serum IgG against the structural proteins, indicating past infection with B19. NS1‐specific antibodies were detected in sera from 29 patients (39%) and 27 controls (22%) (P < 0.001). In addition, 3 patients (4%) showed VP2‐specific IgM. In 15 patients, viral DNA could be repeatedly detected in followup samples of serum and synovial fluid. Sequencing revealed low‐degree nucleotide variations that are in the range of genotype 1 of parvovirus B19. Immunocomplexed virus was present in varying amounts, both in the sera and in the synovial fluid samples. Conclusion Parvovirus B19 is frequently found in serum or synovial fluid of children with rheumatism. The rate of persistent B19 infection in these patients is significantly higher than in age‐matched controls.

Journal

Arthritis & Rheumatology – Wiley

Published: Jun 1, 2003

References

  • Parvovirus infections in children and adults
    Cherry, Cherry
  • Chronic bone marrow failure due to persistent parvovirus B19 infection
    Kurtzman, Kurtzman; Ozawa, Ozawa; Cohen, Cohen; Hanson, Hanson; Oseas, Oseas; Blase, Blase
  • Recurrent granulocytic aplasia as clinical presentation of a persistent parvovirus B19 infection
    Pont, Pont; Puchhammer‐Stöckl, Puchhammer‐Stöckl; Chott, Chott; Popow‐Kraupp, Popow‐Kraupp; Kienzer, Kienzer; Postner, Postner
  • Characterisation of capsid and noncapsid proteins of B19 parvovirus propagated in human erythroid bone marrow cell cultures
    Ozawa, Ozawa; Young, Young
  • Persistence of parvovirus B19 DNA in synovial membranes of young patients with and without chronic arthropathy
    Söderlund, Söderlund; von Essen, von Essen; Haapasaari, Haapasaari; Kiistala, Kiistala; Kiviluoto, Kiviluoto; Hedman, Hedman
  • Presence and significance of human parvovirus B19 DNA in synovial membranes and bone marrow from patients with arthritis of unknown origin
    Cassinotti, Cassinotti; Siegl, Siegl; Michel, Michel; Bruhlmann, Bruhlmann
    Bookmark
  • A viral phospholipase A2 is required for parvovirus infectivity
    Zadori, Zadori; Szelei, Szelei; Lacoste, Lacoste; Li, Li; Gariepy, Gariepy; Raymond, Raymond
  • The VP1 unique region of parvovirus B19 and its constituent phospholipase A2‐like activity
    Dorsch, Dorsch; Liebisch, Liebisch; Kaufmann, Kaufmann; Von Landenberg, Von Landenberg; Hoffmann, Hoffmann; Drobnik, Drobnik
  • Immune response to parvovirus B19 and an antibody defect in persistent viral infection
    Kurtzman, Kurtzman; Cohen, Cohen; Hanson, Hanson; Field, Field; Oseas, Oseas; Blase, Blase
  • Pure red cell aplasia of 10 years duration due to persistent parvovirus B19 infection and its cure with immunoglobulin therapy
    Kurtzman, Kurtzman; Frickhofen, Frickhofen; Kimball, Kimball; Jenkins, Jenkins; Nienhuis, Nienhuis; Young, Young
  • Human parvovirus B19 nonstructural protein induces apoptosis in erythroid lineage cells
    Moffat, Moffat; Yaegashi, Yaegashi; Tada, Tada; Tanaka, Tanaka; Sugamura, Sugamura
  • Characterization of cis‐acting and NS1‐responsive elements in the p6 promoter of parvovirus B19
    Gareus, Gareus; Gigler, Gigler; Hemauer, Hemauer; Leruez‐Ville, Leruez‐Ville; Morinet, Morinet; Wolf, Wolf
  • Cellular transcription factors that interact with p6 promoter elements of parvovirus B19
    Raab, Raab; Bauer, Bauer; Gigler, Gigler; Beckenlehner, Beckenlehner; Wolf, Wolf; Modrow, Modrow
  • Detection of antibodies to the non‐structural protein of parvovirus B19 in persistently infected patients: implications for pathogenesis
    Von Poblotzki, Von Poblotzki; Hemauer, Hemauer; Gigler, Gigler; Puchhammer‐Stöckl, Puchhammer‐Stöckl; Heinz, Heinz; Pont, Pont
  • There is no correlation between polymyalgia rheumatica and acute parvovirus B19 infection
    Hemauer, Hemauer; Modrow, Modrow; Georgi, Georgi; Helmke, Helmke; Veith, Veith; Lang, Lang
  • Antibodies to parvovirus B19 non‐structural protein are associated with chronic but not acute arthritis following B19 infection
    Kerr, Kerr; Cuniffe, Cuniffe
  • Persistence of parvovirus B19 DNA in synovium of patients with haemophilic arthritis
    Zakrzewska, Zakrzewska; Azzi, Azzi; De Biasi, De Biasi; Radossi, Radossi; De Santis, De Santis; Davoli, Davoli
  • Chronic human parvovirus B19 infection in rheumatic disease of childhood and adolescence
    Lehmann, Lehmann; Kühner, Kühner; Beckenlehner, Beckenlehner; Müller‐Godeffroy, Müller‐Godeffroy; Heide, Heide; Küster, Küster
  • The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis
    Arnett, Arnett; Edworthy, Edworthy; Bloch, Bloch; McShane, McShane; Fries, Fries; Cooper, Cooper
    Bookmark
  • Revision of the proposed classification criteria for juvenile idiopathic arthritis
    Petty, Petty; Southwood, Southwood; Baum, Baum; Bheattay, Bheattay; Glass, Glass; Manners, Manners
  • Parvovirus B19 infection in pregnancy: quantitative viral DNA analysis using a kinetic fluorescence detection system (TaqMan PCR)
    Knöll, Knöll; Louwen, Louwen; Kochanowski, Kochanowski; Plentz, Plentz; Stüssel, Stüssel; Beckenlehner, Beckenlehner
  • Sequence variability among different parvovirus B19 isolates
    Hemauer, Hemauer; von Poblotzki, von Poblotzki; Gigler, Gigler; Cassinotti, Cassinotti; Siegl, Siegl; Wolf, Wolf
  • Seroprevalence of parvovirus B19 NS1‐specific IgG in B19 infected and uninfected individuals and in infected pregnant women
    Hemauer, Hemauer; Gigler, Gigler; Searle, Searle; Beckenlehner, Beckenlehner; Raab, Raab; Broliden, Broliden
    Bookmark
  • Genetic diversity within human erythroviruses: identification of three genotypes
    Servant, Servant; Laperche, Laperche; Lallemand, Lallemand; Marinho, Marinho; de Saint Maur, de Saint Maur; Meritet, Meritet
  • Evidence for persistence of human parvovirus B19 DNA in bone marrow
    Cassinotti, Cassinotti; Burtonboy, Burtonboy; Fopp, Fopp; Siegl, Siegl
    Bookmark
  • Quantitative evidence for persistence of human parvovirus B19 DNA in an immunocompetent individual
    Cassinotti, Cassinotti; Siegl, Siegl
    Bookmark
  • Association of parvovirus B19 genome in children with myocarditis and cardiac allograft rejection: diagnosis using the polymerase chain reaction
    Schowengerdt, Schowengerdt; Ni, Ni; Denfield, Denfield; Gajarski, Gajarski; Bowles, Bowles; Rosenthal, Rosenthal
  • Human parvovirus B19 infection in infancy associated with acute and chronic lymphocytic myocarditis and high cytokine levels: report of 3 cases and review
    Nigro, Nigro; Bastianon, Bastianon; Colloridi, Colloridi; Ventriglia, Ventriglia; Gallo, Gallo; D'Amati, D'Amati
  • Evidence for persistence of parvovirus B19 DNA in livers of adults
    Eis‐Hübinger, Eis‐Hübinger; Reber, Reber; Abdul‐Nour, Abdul‐Nour; Glatzel, Glatzel; Lauschke, Lauschke; Putz, Putz
  • Human parvovirus B19 and blood products
    Prowse, Prowse; Ludlam, Ludlam; Yap, Yap
  • Human parvovirus B19: prevalence of viral DNA in volunteer blood donors and clinical outcome of transfusion patients
    Jordan, Jordan; Tiangco, Tiangco; Kiss, Kiss; Koch, Koch
  • Screening of blood donors for human parvovirus B19 and characterization of the results
    Wakamatsu, Wakamatsu; Takakura, Takakura; Kojima, Kojima; Kiriyama, Kiriyama; Goto, Goto; Matsumoto, Matsumoto
  • Prevalence of antihuman parvovirus B19 IgG antibodies in patients with refractory rheumatoid arthritis and polyarticular juvenile rheumatoid arthritis
    Mimori, Mimori; Misaki, Misaki; Hachiya, Hachiya; Ito, Ito; Kano, Kano
  • Human parvovirus B19‐associated arthritis in children
    Nocton, Nocton; Miller, Miller; Tucker, Tucker; Schaller, Schaller
  • Persistence of B19 parvovirus in synovial membranes of patients with rheumatoid arthritis
    Saal, Saal; Steidle, Steidle; Einsele, Einsele; Müller, Müller; Fritz, Fritz; Zacher, Zacher
  • High incidence of parvovirus B19 DNA in synovial tissue of patients with undifferentiated mono‐ and oligoarthritis
    Stahl, Stahl; Seidl, Seidl; Hubner, Hubner; Altrichter, Altrichter; Pfeiffer, Pfeiffer; Pustowoit, Pustowoit
    Bookmark
  • Detection of multiple viral DNA species in synovial tissue and fluid of patients with early arthritis
    Stahl, Stahl; Hubner, Hubner; Seidl, Seidl; Liebert, Liebert; van der Heijden, van der Heijden; Wilbrink, Wilbrink
  • Integrity and full coding sequence of B19 virus DNA persisting in human synovial tissue
    Hokynar, Hokynar; Brunstein, Brunstein; Söderlund‐Venermo, Söderlund‐Venermo; Kiviluoto, Kiviluoto; Partio, Partio; Konttinen, Konttinen
  • Induction of an invasive phenotype by human parvovirus B19 in normal human fibroblasts
    Ray, Ray; Nieva, Nieva; Seftor, Seftor; Khalkali‐Ellis, Khalkali‐Ellis; Naides, Naides
    Bookmark
  • Association of acute human parvovirus B19 infection with human leukocyte antigen class I and II alleles
    Kerr, Kerr; Mattey, Mattey; Thomson, Thomson; Poulton, Poulton; Ollier, Ollier

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