Frequent infection with a viral pathogen, parvovirus B19, in rheumatic diseases of childhood

Frequent infection with a viral pathogen, parvovirus B19, in rheumatic diseases of childhood Objective To find further evidence of the association of parvovirus B19 infection with juvenile rheumatic diseases, and to get new insights into the immunopathogenesis of these diseases. Methods Paired serum and synovial fluid samples from 74 children with rheumatic disease were analyzed with respect to their content of viral DNA and antibodies directed against the B19 viral proteins VP1, VP2, and NS1. Control sera from 124 children with noninflammatory bone diseases or growth retardation were also analyzed. The sequence of the viral DNA, amplified by polymerase chain reaction (PCR), was determined. IgG‐complexed virus was isolated from sera and synovial fluid by adsorption to protein A beads. The amount of free virus versus immunocomplexed virus particles was determined by quantification of the viral genomes by quantitative PCR. Results Twenty‐six of the 74 patients (35%) had detectable amounts of parvovirus B19 DNA in the serum (n = 22 (30%)) and/or the synovial fluid (n = 16 (22%)), whereas only 9 of the 124 control sera (7%) were positive for the viral DNA (P < 0.0001). Forty‐six patients (62%) had serum IgG against the structural proteins, indicating past infection with B19. NS1‐specific antibodies were detected in sera from 29 patients (39%) and 27 controls (22%) (P < 0.001). In addition, 3 patients (4%) showed VP2‐specific IgM. In 15 patients, viral DNA could be repeatedly detected in followup samples of serum and synovial fluid. Sequencing revealed low‐degree nucleotide variations that are in the range of genotype 1 of parvovirus B19. Immunocomplexed virus was present in varying amounts, both in the sera and in the synovial fluid samples. Conclusion Parvovirus B19 is frequently found in serum or synovial fluid of children with rheumatism. The rate of persistent B19 infection in these patients is significantly higher than in age‐matched controls. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arthritis & Rheumatology Wiley

Frequent infection with a viral pathogen, parvovirus B19, in rheumatic diseases of childhood

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Publisher
Wiley
Copyright
Copyright © 2003 by the American College of Rheumatology
ISSN
0004-3591
eISSN
1529-0131
D.O.I.
10.1002/art.10979
Publisher site
See Article on Publisher Site

Abstract

Objective To find further evidence of the association of parvovirus B19 infection with juvenile rheumatic diseases, and to get new insights into the immunopathogenesis of these diseases. Methods Paired serum and synovial fluid samples from 74 children with rheumatic disease were analyzed with respect to their content of viral DNA and antibodies directed against the B19 viral proteins VP1, VP2, and NS1. Control sera from 124 children with noninflammatory bone diseases or growth retardation were also analyzed. The sequence of the viral DNA, amplified by polymerase chain reaction (PCR), was determined. IgG‐complexed virus was isolated from sera and synovial fluid by adsorption to protein A beads. The amount of free virus versus immunocomplexed virus particles was determined by quantification of the viral genomes by quantitative PCR. Results Twenty‐six of the 74 patients (35%) had detectable amounts of parvovirus B19 DNA in the serum (n = 22 (30%)) and/or the synovial fluid (n = 16 (22%)), whereas only 9 of the 124 control sera (7%) were positive for the viral DNA (P < 0.0001). Forty‐six patients (62%) had serum IgG against the structural proteins, indicating past infection with B19. NS1‐specific antibodies were detected in sera from 29 patients (39%) and 27 controls (22%) (P < 0.001). In addition, 3 patients (4%) showed VP2‐specific IgM. In 15 patients, viral DNA could be repeatedly detected in followup samples of serum and synovial fluid. Sequencing revealed low‐degree nucleotide variations that are in the range of genotype 1 of parvovirus B19. Immunocomplexed virus was present in varying amounts, both in the sera and in the synovial fluid samples. Conclusion Parvovirus B19 is frequently found in serum or synovial fluid of children with rheumatism. The rate of persistent B19 infection in these patients is significantly higher than in age‐matched controls.

Journal

Arthritis & RheumatologyWiley

Published: Jun 1, 2003

References

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    Cassinotti, Cassinotti; Siegl, Siegl; Michel, Michel; Bruhlmann, Bruhlmann
  • The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis
    Arnett, Arnett; Edworthy, Edworthy; Bloch, Bloch; McShane, McShane; Fries, Fries; Cooper, Cooper
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    Cassinotti, Cassinotti; Burtonboy, Burtonboy; Fopp, Fopp; Siegl, Siegl
  • Quantitative evidence for persistence of human parvovirus B19 DNA in an immunocompetent individual
    Cassinotti, Cassinotti; Siegl, Siegl
  • High incidence of parvovirus B19 DNA in synovial tissue of patients with undifferentiated mono‐ and oligoarthritis
    Stahl, Stahl; Seidl, Seidl; Hubner, Hubner; Altrichter, Altrichter; Pfeiffer, Pfeiffer; Pustowoit, Pustowoit
  • Induction of an invasive phenotype by human parvovirus B19 in normal human fibroblasts
    Ray, Ray; Nieva, Nieva; Seftor, Seftor; Khalkali‐Ellis, Khalkali‐Ellis; Naides, Naides

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