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A. Hijazi, M. Devonec, R. Bouvier, P. Bringuier, J. Revillard (1991)
Use of leukocytes infiltrating bladder tumors as an internal reference for determining the DNA index by flow cytometry.Analytical and quantitative cytology and histology, 13 2
S. Khochbin, A. Chabanas, P. Albert, J. Albert, J. Lawrence (1988)
Application of bromodeoxyuridine incorporation measurements to the determination of cell distribution within the S phase of the cell cycle.Cytometry, 9 5
A. Hurley (1988)
Quality control in flow cytometry.Cytometry. Supplement : the journal of the Society for Analytical Cytology, 3
F. Klein, F. White (1988)
Flow cytometry deoxyribonucleic acid determinations and cytology of bladder washings: practical experience.The Journal of urology, 139 2
L. Koss, R. Wersto, Deborah Simmons, D. Deitch, F. Herz, S. Freed (1989)
Predictive value of DNA measurements in bladder washings. Comparison of flow cytometry, image cytophotometry, and cytology in patients with a past history of urothelial tumorsCancer, 64
W. Hiddemann, J. Schumann, M. Andreeff, B. Barlogie, Chester Herman, R. Leif, B. Mayall, R. Murphy, A. Sandberg (1984)
Convention on nomenclature for DNA cytometryCancer Genetics and Cytogenetics, 13
Y Fradet, M Tardif, L Bourget, J Robert (1990)
Clinical cancer progression in urinary bladder tumors evaluated by multiparameter flow cytometry with monoclonal antibodies, 50
O. Frankfurt, R. Huben (1984)
Clinical applications of DNA flow cytometry for bladder tumors.Urology, 23 3 Suppl
HJ Tanke, AMJ Van Driel‐Kulker, CJ Cornelisse, JS Ploem (1983)
Combined flow and image cytometry of the same cytological sample, 130
P. Bretton, A. Myc, C. Cordon-Cardo, P. DeAngelis, W. Fair, M. Melamed (1989)
Initial evaluation of a new epithelial antigen (T16) for bivariate flow cytometry of bladder irrigation specimens.Cytometry, 10 3
L. Wheeless, J. Reeder, M. O'connell, R. Robinson, J. Cosgriff, Y. Fradet, I. Frank, A. Cockett (1991)
DNA slit-scan flow cytometry of bladder irrigation specimens and the importance of recognizing urothelial cells.Cytometry, 12 2
P Vielh, H Magdelenat, Y Remvikos, B Dutrillaux (1991)
Flow Cytometry: Guides to Clinical Aspiration Biopsy
W. Feitz, H. Beck, A. Smeets, F. Debruyne, G. Vooijs, C. Herman, F. Ramaekers (1985)
TISSUE‐SPECIFIC MARKERS IN FLOW CYTOMETRY OF UROLOGICAL CANCERS: CYTOKERATINS IN BLADDER CARCINOMAInternational Journal of Cancer, 36
Y. Fradet, M. Tardif, L. Bourget, J. Robert (1990)
Clinical cancer progression in urinary bladder tumors evaluated by multiparameter flow cytometry with monoclonal antibodies. Laval University Urology Group.Cancer research, 50 2
J. Huffman, P. Garin‐Chesa, H. Gay, W. Whitmore, M. Melamed (1986)
Flow Cytometric Identification of Human Bladder Cells Using a Cytokeratin Monoclonal Antibody aAnnals of the New York Academy of Sciences, 468
L. Collste, Z. Darżynkiewicz, F. Traganos, T. Sharpless, W. Whitmore, M. Melamed (1979)
Identification of polymorphonuclear leukocytes in cytologic samples for flow cytometry.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 27
G Brugal (1984)
Methods and Achievements in Experimental Pathology
H. Tanke, A. Driel‐Kulker, C. Cornelisse, J. Ploem (1983)
Combined flow cytometry and image cytometry of the same cytological sampleJournal of Microscopy, 130
M Devonec, A Hijazi (1988)
La cytométrie en flux pour l'étude de la cellule normale ou pathologique
MR Melamed, L Staiano‐Coico (1990)
Flow Cytometry and Sorting
Y. Fradet, C. Cordon-Cardo, W. Whitmore, M. Melamed, L. Old (1986)
Cell surface antigens of human bladder tumors: definition of tumor subsets by monoclonal antibodies and correlation with growth characteristics.Cancer research, 46 10
Using flow or image cytometry, we compared the DNA distribution of cells from bladder washings from 52 patients with bladder cancer. For image cytometry, urothelial nuclei (recognized visually) were analyzed for DNA content using polymorphonuclear nuclei as internal diploid reference. For flow cytometry, two methods can be used: either all cells can be analyzed, as commonly performed, or urothelial cells can be analyzed alone, after specific derection. In this flow cytometry study, cells were doubly stained for panurothelial antigen T16 and for DNA. All the cells were first analyzed using peripheral lymphocytes as an external reference; 79% of the results were similar with results obtained from image analysis. For discordant results, flow‐cytometric data were reprocessed to identify immunologically stained urothelial cells; one additional case became concordant with image cytometry when only urothelial cells were analyzed, with lymphocytes as diploid reference; a better concordance (94%) was found when the nonurothelial cells of the samples served as a diploid reference instead of peripheral lymphocytes. This suggests that we achieved an improvement of the flow‐cytometric evaluations for ploidy assessement, and we conclude that, on these conditions, flow or image analysis can be considered as equivalent methods for DNA content studies of tumors. © 1993 Wiley‐Liss, Inc.
Cytometry Part A – Wiley
Published: Nov 1, 1993
Keywords: ; ;
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