Ficoll‐hypaque separation vs whole blood lysis: Comparison of efficiency and impact on minimal residual disease analysis

Ficoll‐hypaque separation vs whole blood lysis: Comparison of efficiency and impact on minimal... INTRODUCTIONThe studies of the latest years highlighted how molecular biology laboratory practice changed from single to high‐throughput analysis, accompanied by an increase in the number of samples tested simultaneously.In the high‐throughput era, also the pre‐analytical phase requires the automation of the experiment setting and especially the methodology of nucleic acid extraction. Nevertheless, the current biobanking procedures and cell recovery are still labor‐intensive, requiring long time and dedicated laboratory staff.In many hematological clinical trials, as well as in the clinical practice of selected centers, bone marrow (BM) and peripheral blood (PB) samples are being collected and analyzed at diagnosis and during the treatment phases for minimal residual disease (MRD) and mutational studies. MRD analysis by polymerase chain reaction (PCR) has been shown to be a powerful early predictor of therapy response and outcome in lymphoproliferative disorders, such as acute lymphoblastic leukemia, follicular lymphoma (FL), mantle cell lymphoma (MCL), and multiple myeloma (MM).In previous published studies, MRD analysis was commonly performed on genomic DNA (gDNA) from BM or PB mononuclear cells (MNCs) collected using density gradient separation approach. Since the 80s, Ficoll, a sucrose polymer with high molecular weight (1077 g/mL), has been recommended to collect MNCs. This is a feasible procedure, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Laboratory Hematology Wiley
Loading next page...
 
/lp/wiley/ficoll-hypaque-separation-vs-whole-blood-lysis-comparison-of-GPEqrul7ys
Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 John Wiley & Sons Ltd
ISSN
1751-5521
eISSN
1751-553X
D.O.I.
10.1111/ijlh.12766
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONThe studies of the latest years highlighted how molecular biology laboratory practice changed from single to high‐throughput analysis, accompanied by an increase in the number of samples tested simultaneously.In the high‐throughput era, also the pre‐analytical phase requires the automation of the experiment setting and especially the methodology of nucleic acid extraction. Nevertheless, the current biobanking procedures and cell recovery are still labor‐intensive, requiring long time and dedicated laboratory staff.In many hematological clinical trials, as well as in the clinical practice of selected centers, bone marrow (BM) and peripheral blood (PB) samples are being collected and analyzed at diagnosis and during the treatment phases for minimal residual disease (MRD) and mutational studies. MRD analysis by polymerase chain reaction (PCR) has been shown to be a powerful early predictor of therapy response and outcome in lymphoproliferative disorders, such as acute lymphoblastic leukemia, follicular lymphoma (FL), mantle cell lymphoma (MCL), and multiple myeloma (MM).In previous published studies, MRD analysis was commonly performed on genomic DNA (gDNA) from BM or PB mononuclear cells (MNCs) collected using density gradient separation approach. Since the 80s, Ficoll, a sucrose polymer with high molecular weight (1077 g/mL), has been recommended to collect MNCs. This is a feasible procedure,

Journal

International Journal of Laboratory HematologyWiley

Published: Jan 1, 2018

Keywords: ; ; ;

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off