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Jiaojiao Zhang, W. Talbot, A. Schier (1998)
Positional Cloning Identifies Zebrafish one-eyed pinhead as a Permissive EGF-Related Ligand Required during GastrulationCell, 92
Gary Stuart, Juergen Elkind, James McMURRAY, Monte, Westerfield (1990)
Stable lines of transgenic zebrafish exhibit reproducible patterns of transgene expression.Development, 109 3
G. Stuart, James McMURRAY, M. Westerfield (1988)
Replication, integration and stable germ-line transmission of foreign sequences injected into early zebrafish embryos.Development, 103 2
Z. Gong (1999)
Zebrafish expressed sequence tags and their applications.Methods in cell biology, 60
S. Higashijima, H. Okamoto, N. Ueno, Yoshiki Hotta, G. Eguchi (1997)
High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using promoters of zebrafish origin.Developmental biology, 192 2
Z. Gong, T. Yan, J. Liao, Sze Lee, Jiangyan He, C. Hew (1997)
Rapid identification and isolation of zebrafish cDNA clones.Gene, 201 1-2
F. Müller, Darren Williams, J. Kobolák, L. Gauvry, G. Goldspink, László Orbán, N. Maclean (1997)
Activator effect of coinjected enhancers on the muscle‐specific expression of promoters in zebrafish embryosMolecular Reproduction and Development, 47
S. Efrat, Susanne Linde, H. Kofod, D. Spector, Michael. Delannoy, S. Grant, D. Hanahan, S. Baekkeskov (1988)
Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene.Proceedings of the National Academy of Sciences of the United States of America, 85 23
A Iyengar, F Muller, N Maclean (1996)
Regulation and expression of transgenes in fish— a review, 5
J. Liao, C. Chan, Z. Gong (1997)
An alternative linker-mediated polymerase chain reaction method using a dideoxynucleotide to reduce amplification background.Analytical biochemistry, 253 1
F. Grosveld, Greet Assendelft, D. Greaves, G. Kollias (1987)
Position-independent, high-level expression of the human β-globin gene in transgenic miceCell, 51
Q. Long, A. Meng, Han Wang, J. Jessen, Michaela Farrell, Shuo Lin (1997)
GATA-1 expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene.Development, 124 20
V. Korzh, I. Sleptsova, J. Liao, Jiangyan He, Z. Gong (1998)
Expression of zebrafish bHLH genes ngn1and nrd defines distinct stages of neural differentiationDevelopmental Dynamics, 213
M. Westerfield (1995)
The zebrafish book : a guide for the laboratory use of zebrafish (Danio rerio)
Robert Mcknight, Avi Shamay, Lakshmanan Sankaran, Robert Wall, Lothar Hennighausen (1992)
Matrix-attachment regions can impart position-independent regulation of a tissue-specific gene in transgenic mice.Proceedings of the National Academy of Sciences of the United States of America, 89 15
Shuo Lin, Samuel Yang, N. Hopkins (1994)
lacZ expression in germline transgenic zebrafish can be detected in living embryos.Developmental biology, 161 1
A. Brownlie, A. Donovan, S. Pratt, B. Paw, B. Paw, A. Oates, C. Brugnara, Witkowska He, S. Sassa, Leonard Zon, Leonard Zon (1998)
Positional cloning of the zebrafish sauternes gene: a model for congenital sideroblastic anaemiaNature Genetics, 20
J. Postlethwait, L. Johnson, N. Midson, W. Talbot, Michael Gates, W. Ballinger, Dana Africa, Rebecca Andrews, Tim Carl, S. Eisen, S. Horne, C. Kimmel, Mark Hutchinson, Michele Johnson, Andre Rodriguez (1994)
A genetic linkage map for the zebrafish.Science, 264 5159
M. Westerfield, J. Wegner, B. Jegalian, E. DeRobertis, A. Püschel (1992)
Specific activation of mammalian Hox promoters in mosaic transgenic zebrafish.Genes & development, 6 4
W. Driever, L. Solnica-Krezel, A. Schier, S. Neuhauss, J. Malicki, D. Stemple, D. Stainier, F. Zwartkruis, S. Abdelilah, Z. Rangini, J. Belak, C. Boggs (1996)
A genetic screen for mutations affecting embryogenesis in zebrafish.Development, 123
J. Postlethwait, Yi-Lin Yan, M. Gates, S. Horne, A. Amores, A. Brownlie, A. Donovan, Elizabeth Egan, A. Force, Z. Gong, C. Goutel, A. Fritz, R. Kelsh, E. Knapik, Eric Liao, B. Paw, D. Ransom, A. Singer, Margaret Thomson, T. Abduljabbar, P. Yelick, Dave Beier, J. Joly, D. Larhammar, F. Rosa, M. Westerfield, L. Zon, Steve Johnson, W. Talbot (1998)
Vertebrate genome evolution and the zebrafish gene mapNature Genetics, 18
E. Knapik, A. Goodman, M. Ekker, M. Chevrette, Jonathan Delgado, S. Neuhauss, N. Shimoda, W. Driever, M. Fishman, H. Jacob (1998)
A microsatellite genetic linkage map for zebrafish (Danio rerio)Nature Genetics, 18
BP Cormack, RH Valdivia, S Falkow (1995)
FACS‐optimized mutants of the green fluorescent protein (GFP), 173
P. Haffter, M. Granato, M. Brand, M. Mullins, M. Hammerschmidt, D. Kane, J. Odenthal, F. Eeden, Yun-Jin Jiang, C. Heisenberg, R. Kelsh, M. Furutani-Seiki, E. Vogelsang, D. Beuchle, Ursula Schach, Cosima Fabian, C. Nüsslein-Volhard (1996)
The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio.Development, 123
Yi-Lin Yan, William Talbot, Elizabeth Egan, J. Postlethwait (1998)
Mutant rescue by BAC clone injection in zebrafish.Genomics, 50 2
Raymond MacDonald, G. Swift (1998)
Analysis of transcriptional regulatory regions in vivo.The International journal of developmental biology, 42 7
A. Amsterdam, Shuo Lin, Nancy Hopkins (1995)
The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos.Developmental biology, 171 1
M Westerfield (1994)
The zebrafish book. A guide for the laboratory use of zebrafish (Brachydanio rerio)
Z Gong, CL Hew (1995)
Transgenic fish in aquaculture and developmental biology, 30
PB Hackett (1993)
Biochemistry and molecular biology of fishes. 2. Molecular biology frontiers
Anming Meng, Hong Tang, B. Ong, Michael Farrell, Shuo Lin (1997)
Promoter analysis in living zebrafish embryos identifies a cis-acting motif required for neuronal expression of GATA-2.Proceedings of the National Academy of Sciences of the United States of America, 94 12
Although the zebrafish has become a popular model organism for vertebrate developmental and genetic analyses, its use in transgenic studies still suffers from the scarcity of homologous gene promoters. In the present study, three different zebrafish cDNA clones were isolated and sequenced completely, and their expression patterns were characterized by whole‐mount in situ hybridization as well as by Northern blot hybridization. The first clone encodes a type II cytokeratin (CK), which is specifically expressed in skin epithelia in early embryos and prominently expressed in the adult skin tissue. The second clone is muscle specific and encodes a muscle creatine kinase (MCK). The third clone, expressed ubiquitously in all tissues, is derived from an acidic ribosomal phosphoprotein P0 (arp) gene. In order to test the fidelity of zebrafish embryos in transgenic expression, the promoters of the three genes were isolated using a rapid linker‐mediated PCR approach and subsequently ligated to a modified green fluorescent protein (gfp) reporter gene. When the three hybrid GFP constructs were introduced into zebrafish embryos by microinjection, the three promoters were activated faithfully in developing zebrafish embryos. The 2.2‐kb ck promoter was sufficient to direct GFP expression in skin epithelia, although a weak expression in muscle was also observed in a few embryos. This pattern of transgenic expression is consistent with the expression pattern of the endogenous cytokeratin gene. The 1.5‐kb mck promoter/gfp was expressed exclusively in skeletal muscles and not elsewhere. By contrast, the 0.8‐kb ubiquitous promoter plus the first intron of the arp gene were capable of expressing GFP in a variety of tissues, including the skin, muscle, lens, neurons, notochord, and circulating blood cells. Our experiments, therefore, further demonstrated that zebrafish embryos can faithfully express exogenously introduced genes under the control of zebrafish promoters. Dev. Genet. 25:158–167, 1999. © 1999 Wiley‐Liss, Inc.
Genesis: the Journal of Genetics and Development – Wiley
Published: Jan 1, 1999
Keywords: ; ; ; ; ;
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