Expression of the mRNA of Seven Metabotropic Glutamate Receptors (mGluR1 to 7) in the Rat Retina. An In Situ Hybridization Study on Tissue Sections and Isolated Cells

Expression of the mRNA of Seven Metabotropic Glutamate Receptors (mGluR1 to 7) in the Rat Retina.... We have studied the expression of mRNAs for seven metabotropic glutamate receptors (mGluR1–7) in the retina of the adult rat by in situ hybridization with tissue sections and isolated cells using (α35S)dATP‐labelled oligonucleotide probes. Hybridization revealed the expression of six of the metabotropic receptor mRNAs, mGluR1, 2 and 4–7, in the retina, while mGluR3 was not detected. Each of the expressed receptor mRNAs showed a distinct pattern of expression. In the outer nuclear layer, corresponding to photoreceptor somata, no labelling was detected. In the outer part of the inner nuclear layer, putative horizontal cells were labelled for mGluR5. More proximal in this layer, corresponding to the position of bipolar cell somata, there was strong labelling for mGluR6. A small number of bipolar cells were also labelled for mGluR5 and mGluR7. In situ hybridization with isolated cells showed that mGluR6 was expressed by rod bipolar cells. Subsets of amacrine cells, with cell bodies along the border between the inner nuclear layer and the inner plexiform layer, were positive for mGluR1, 2, 4 and 7, suggesting considerable heterogeneity of these receptors among amacrine cells. None of the seven metabotropic receptor mRNAs was expressed in isolated Müller glial cells. In the ganglion cell layer, virtually every ganglion cell and displaced amacrine cell was labelled for mGluR1 and mGluR4. Some cells in this layer (˜20% of the total), most likely both ganglion cells and displaced amacrine cells, were also labelled for mGluR2 and mGluR7. These findings suggest that metabotropic glutamate receptors are considerably more widespread among neurons in the retina than indicated by previous physiological and pharmacological investigations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Neuroscience Wiley

Expression of the mRNA of Seven Metabotropic Glutamate Receptors (mGluR1 to 7) in the Rat Retina. An In Situ Hybridization Study on Tissue Sections and Isolated Cells

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Publisher
Wiley
Copyright
Copyright © 1995 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0953-816X
eISSN
1460-9568
DOI
10.1111/j.1460-9568.1995.tb01142.x
Publisher site
See Article on Publisher Site

Abstract

We have studied the expression of mRNAs for seven metabotropic glutamate receptors (mGluR1–7) in the retina of the adult rat by in situ hybridization with tissue sections and isolated cells using (α35S)dATP‐labelled oligonucleotide probes. Hybridization revealed the expression of six of the metabotropic receptor mRNAs, mGluR1, 2 and 4–7, in the retina, while mGluR3 was not detected. Each of the expressed receptor mRNAs showed a distinct pattern of expression. In the outer nuclear layer, corresponding to photoreceptor somata, no labelling was detected. In the outer part of the inner nuclear layer, putative horizontal cells were labelled for mGluR5. More proximal in this layer, corresponding to the position of bipolar cell somata, there was strong labelling for mGluR6. A small number of bipolar cells were also labelled for mGluR5 and mGluR7. In situ hybridization with isolated cells showed that mGluR6 was expressed by rod bipolar cells. Subsets of amacrine cells, with cell bodies along the border between the inner nuclear layer and the inner plexiform layer, were positive for mGluR1, 2, 4 and 7, suggesting considerable heterogeneity of these receptors among amacrine cells. None of the seven metabotropic receptor mRNAs was expressed in isolated Müller glial cells. In the ganglion cell layer, virtually every ganglion cell and displaced amacrine cell was labelled for mGluR1 and mGluR4. Some cells in this layer (˜20% of the total), most likely both ganglion cells and displaced amacrine cells, were also labelled for mGluR2 and mGluR7. These findings suggest that metabotropic glutamate receptors are considerably more widespread among neurons in the retina than indicated by previous physiological and pharmacological investigations.

Journal

European Journal of NeuroscienceWiley

Published: Jul 1, 1995

References

  • Agonists at metabotropic glutamate receptors presynaptically inhibit EPSCs in neonatal rat hippocampus
    Baskys, Baskys; Malenka, Malenka
  • Expression of NMDA and high‐affinity kainate receptor subunit mRNAs in the adult rat retina
    Brandstätter, Brandstätter; Hartveit, Hartveit; Sassoè‐Pognetto, Sassoè‐Pognetto; Wässle, Wässle
  • Activation of a large‐conductance Ca 2+ ‐dependent K + channel by stimulation of glutamate phosphoinositide‐coupled receptors in cultured cerebellar granule cells
    Fagni, Fagni; Bossu, Bossu; Bockaert, Bockaert
  • Reduction of resting K + current by metabotropic glutamate and muscarinic receptors in rat CA3 cells: mediation by G‐proteins
    Guérineau, Guérineau; Gähwiler, Gähwiler; Gerber, Gerber
  • Cloned glutamate receptors
    Hollmann, Hollmann; Heinemann, Heinemann
  • Excitatory amino acid receptors of rod‐ and cone‐driven horizontal cells in the rabbit retina
    Massey, Massey; Miller, Miller
  • The excitatory amino acid receptors: their classes, pharmacology, and distinct properties in the function of the central nervous system
    Monaghan, Monaghan; Bridges, Bridges; Cotman, Cotman
  • Pharmacological modulation of the rod pathway in the cat retina
    Müller, Müller; Wässle, Wässle; Voigt, Voigt
  • Inhibition of cyclic AMP formation by a selective metabotropic glutamate receptor agonist
    Schoepp, Schoepp; Johnson, Johnson; Monn, Monn
  • APB suppresses synaptic input to retinal horizontal cells in fish: a direct action on horizontal cells modulated by intracellular pH
    Takahashi, Takahashi; Copenhagen, Copenhagen
  • Pharmacological similarity between the retinal APB receptor and the family of metabotropic glutamate receptors
    Tian, Tian; Slaughter, Slaughter
  • Functional architecture of the mammalian retina
    Wässle, Wässle; Boycott, Boycott

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