Expression of NR1 and NR2A/B subunits of the NMDA receptor in cortical astrocytes

Expression of NR1 and NR2A/B subunits of the NMDA receptor in cortical astrocytes Ionotropic glutamate (Glu) receptors of the N‐methyl‐D‐aspartate type (NMDA) play a fundamental role in many cortical functions. Native NMDA receptors are composed of a heteromeric assembly of different subunits belonging to two classes: NMDAR1 (NR1) and NMDAR2 (NR2). To date, NMDA receptors are believed to be expressed only in neurons, although electrophysiological and in situ hybridization studies have suggested that this class of Glu receptors might be also expressed by some astrocytes. In this study, we have investigated in the cerebral cortex of adult rats the presence of astrocytes expressing NR1 and NR2A/B subunits by immunocytochemistry with specific antibodies, and we show that some distal astrocytic processes, but only rarely astrocytic cell bodies, contain immunoreaction product indicative of NR1 and NR2A/B expression. These findings suggest that at least part of the role NMDA has in cortical functions might depend on the activation of astrocytic NMDA receptors; the subcellular localization of NR1 and NR2A/B subunits in distal processes suggests that NMDA receptors contribute to monitoring Glu levels in the extracellular space. © 1996 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Glia Wiley

Expression of NR1 and NR2A/B subunits of the NMDA receptor in cortical astrocytes

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Publisher
Wiley
Copyright
Copyright © 1996 Wiley‐Liss, Inc.
ISSN
0894-1491
eISSN
1098-1136
D.O.I.
10.1002/(SICI)1098-1136(199607)17:3<254::AID-GLIA7>3.0.CO;2-0
Publisher site
See Article on Publisher Site

Abstract

Ionotropic glutamate (Glu) receptors of the N‐methyl‐D‐aspartate type (NMDA) play a fundamental role in many cortical functions. Native NMDA receptors are composed of a heteromeric assembly of different subunits belonging to two classes: NMDAR1 (NR1) and NMDAR2 (NR2). To date, NMDA receptors are believed to be expressed only in neurons, although electrophysiological and in situ hybridization studies have suggested that this class of Glu receptors might be also expressed by some astrocytes. In this study, we have investigated in the cerebral cortex of adult rats the presence of astrocytes expressing NR1 and NR2A/B subunits by immunocytochemistry with specific antibodies, and we show that some distal astrocytic processes, but only rarely astrocytic cell bodies, contain immunoreaction product indicative of NR1 and NR2A/B expression. These findings suggest that at least part of the role NMDA has in cortical functions might depend on the activation of astrocytic NMDA receptors; the subcellular localization of NR1 and NR2A/B subunits in distal processes suggests that NMDA receptors contribute to monitoring Glu levels in the extracellular space. © 1996 Wiley‐Liss, Inc.

Journal

GliaWiley

Published: Jul 1, 1996

References

  • Excitatory amino acid receptors in glia: Different subtypes for distinct function?
    Gallo, Gallo; Russell, Russell
  • Structure and function of the NMDA receptor channel
    Mori, Mori; Mishina, Mishina
  • Activation of glutamate receptors and glutamate uptake in identified macroglial cells in rat cerebellar cultures
    Wyllie, Wyllie; Mathie, Mathie; Simmonds, Simmonds; Cull‐Candy, Cull‐Candy

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