Evidence that actin filaments are involved in controlling the permeability of plasmodesmata in tobacco mesophyll

Evidence that actin filaments are involved in controlling the permeability of plasmodesmata in... The role of actin filaments in regulating plasmodesmal transport has been studied by microinjection experiments in mesophyll cells of tobacco (Nicotiana tabacum L. cv. Samsun). When fluorescent dextrans of various molecular sizes were each co‐injected with specific actin filament perturbants cytochalasin D (CD) or profilin into these cells, dextrans up to 20 kilodalton (kDa) moved from the injected cell into surrounding cells within 3–5 min. In contrast, when such dextrans were injected alone or co‐injected with phalloidin into the mesophyll cells, they remained in the injected cells. Phalloidin co‐injection slowed down or even inhibited CD‐ or profilin‐elicited dextran cell‐to‐cell movement. Dextrans of 40 kDa or larger were unable to move out of the injected cell in the presence of CD or profilin. These data suggest that actin filaments may participate in the regulation of plasmodesmal transport by controlling the permeability of plasmodesmata. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Plant Journal Wiley

Evidence that actin filaments are involved in controlling the permeability of plasmodesmata in tobacco mesophyll

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Publisher
Wiley
Copyright
Copyright © 1996 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0960-7412
eISSN
1365-313X
D.O.I.
10.1046/j.1365-313X.1996.10010157.x
Publisher site
See Article on Publisher Site

Abstract

The role of actin filaments in regulating plasmodesmal transport has been studied by microinjection experiments in mesophyll cells of tobacco (Nicotiana tabacum L. cv. Samsun). When fluorescent dextrans of various molecular sizes were each co‐injected with specific actin filament perturbants cytochalasin D (CD) or profilin into these cells, dextrans up to 20 kilodalton (kDa) moved from the injected cell into surrounding cells within 3–5 min. In contrast, when such dextrans were injected alone or co‐injected with phalloidin into the mesophyll cells, they remained in the injected cells. Phalloidin co‐injection slowed down or even inhibited CD‐ or profilin‐elicited dextran cell‐to‐cell movement. Dextrans of 40 kDa or larger were unable to move out of the injected cell in the presence of CD or profilin. These data suggest that actin filaments may participate in the regulation of plasmodesmal transport by controlling the permeability of plasmodesmata.

Journal

The Plant JournalWiley

Published: Jul 1, 1996

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