Evidence for persistence of human parvovirus B19 DNA in bone marrow

Evidence for persistence of human parvovirus B19 DNA in bone marrow A nested polymerase chain reaction assay (nPCR) was used to investigate the potential of human parvovirus B19 DNA to persist in blood or bone marrow samples obtained either from blood donors or cadaveric bone donors or from patients presenting with clinical signs of parvovirus B19 infection. The presence of parvovirus B19 specific antibody in blood was tested by enzyme immunoassay (EIA). B19 virus genome was not detected in any blood sample of 115 blood donors, of whom 92 (80%) had anti‐B19 IgG antibody only as an indication of past infection. In contrast, B19 virus DNA was detected in the bone marrow of 4 out of 45 bone donors. Each one of the serum samples available for 3 of these 4 individuals contained anti‐B19 IgG antibody. Among 84 patients with clinical manifestations of parvovirus B19 infection, 17 (20%) had B19 virus DNA in bone marrow. Eight of the latter patients had anti‐B19 IgG antibody in their blood but neither anti‐B19 IgM nor B19 virus DNA. These data document the ability of parvovirus B19 DNA to persist in the bone marrow of asymptomatic individuals and patients with parvovirus B19 infection suspected on clinical grounds. J. Med. Virol. 53:229–232, 1997. © 1997 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Medical Virology Wiley

Evidence for persistence of human parvovirus B19 DNA in bone marrow

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Publisher
Wiley
Copyright
Copyright © 1997 Wiley‐Liss, Inc.
ISSN
0146-6615
eISSN
1096-9071
D.O.I.
10.1002/(SICI)1096-9071(199711)53:3<229::AID-JMV8>3.0.CO;2-A
Publisher site
See Article on Publisher Site

Abstract

A nested polymerase chain reaction assay (nPCR) was used to investigate the potential of human parvovirus B19 DNA to persist in blood or bone marrow samples obtained either from blood donors or cadaveric bone donors or from patients presenting with clinical signs of parvovirus B19 infection. The presence of parvovirus B19 specific antibody in blood was tested by enzyme immunoassay (EIA). B19 virus genome was not detected in any blood sample of 115 blood donors, of whom 92 (80%) had anti‐B19 IgG antibody only as an indication of past infection. In contrast, B19 virus DNA was detected in the bone marrow of 4 out of 45 bone donors. Each one of the serum samples available for 3 of these 4 individuals contained anti‐B19 IgG antibody. Among 84 patients with clinical manifestations of parvovirus B19 infection, 17 (20%) had B19 virus DNA in bone marrow. Eight of the latter patients had anti‐B19 IgG antibody in their blood but neither anti‐B19 IgM nor B19 virus DNA. These data document the ability of parvovirus B19 DNA to persist in the bone marrow of asymptomatic individuals and patients with parvovirus B19 infection suspected on clinical grounds. J. Med. Virol. 53:229–232, 1997. © 1997 Wiley‐Liss, Inc.

Journal

Journal of Medical VirologyWiley

Published: Nov 1, 1997

References

  • Human parvovirus‐B19 infections: routine diagnosis by a new nested polymerase chain reaction assay
    Cassinotti, Cassinotti; Weitz, Weitz; Siegl, Siegl
  • Pure red‐cell aplasia: A review
    Erslev, Erslev; Soltan, Soltan

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