Evidence for Glucocorticoid Target Cells in the Rat Optic Nerve. Physicochemical Characterization of Cytosol Binding Sites

Evidence for Glucocorticoid Target Cells in the Rat Optic Nerve. Physicochemical Characterization... Abstract: Cytosolic dexamethasone (DEX) binding sites were studied in the Wallerian‐degenerating rat optic nerve (ON), a tissue that is rich in neuroglial cells but devoid of neuronal perikarya and processes. For comparison, hippocampal (HI) and anterior pituitary (AP) cytosols were studied in parallel. Binding sites in these three tissues were found to be quite similar in almost all respects. The sites have a high affinity for DEX (KD= 2.5–3.5 nM), are present at a high concentration (Bmax= 360–365 fmol/mg cytosol protein), and possess a binding specificity typical of glucocorticoid receptors in other organs. Most experiments supported the assumption of a single DEX‐binding species in each tissue. Saturation analyses consistently yielded linear Scatchard plots over the range of DEX concentrations tested. Density gradient centrifugation in each case revealed a single peak with a sedimentation coefficient of 7–8S at low ionic strength and 4–4.5S in the presence of 0.3 M KCl. Isoelectric focusing similarly localized most of the binding in each cytosol to a single large peak with an isoelectric point of approximately 6.0. Dissociation rate determinations, on the other hand, suggested the possibility of two different binding sites in each tissue. These studies show that glucocorticoid binders present in cells of the ON possess the same characteristics as the cytoplasmic receptors found in HI, AP, and other recognized glucocorticoid target tissues. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

Evidence for Glucocorticoid Target Cells in the Rat Optic Nerve. Physicochemical Characterization of Cytosol Binding Sites

Loading next page...
 
/lp/wiley/evidence-for-glucocorticoid-target-cells-in-the-rat-optic-nerve-SZyVYYhNOX
Publisher
Wiley
Copyright
Copyright © 1982 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
D.O.I.
10.1111/j.1471-4159.1982.tb03964.x
Publisher site
See Article on Publisher Site

Abstract

Abstract: Cytosolic dexamethasone (DEX) binding sites were studied in the Wallerian‐degenerating rat optic nerve (ON), a tissue that is rich in neuroglial cells but devoid of neuronal perikarya and processes. For comparison, hippocampal (HI) and anterior pituitary (AP) cytosols were studied in parallel. Binding sites in these three tissues were found to be quite similar in almost all respects. The sites have a high affinity for DEX (KD= 2.5–3.5 nM), are present at a high concentration (Bmax= 360–365 fmol/mg cytosol protein), and possess a binding specificity typical of glucocorticoid receptors in other organs. Most experiments supported the assumption of a single DEX‐binding species in each tissue. Saturation analyses consistently yielded linear Scatchard plots over the range of DEX concentrations tested. Density gradient centrifugation in each case revealed a single peak with a sedimentation coefficient of 7–8S at low ionic strength and 4–4.5S in the presence of 0.3 M KCl. Isoelectric focusing similarly localized most of the binding in each cytosol to a single large peak with an isoelectric point of approximately 6.0. Dissociation rate determinations, on the other hand, suggested the possibility of two different binding sites in each tissue. These studies show that glucocorticoid binders present in cells of the ON possess the same characteristics as the cytoplasmic receptors found in HI, AP, and other recognized glucocorticoid target tissues.

Journal

Journal of NeurochemistryWiley

Published: Aug 1, 1982

References

  • Hormonal control of glycerolphosphate dehydrogenase in the rat brain
    Vellis, Vellis; Inglish, Inglish
  • Evidence for glucocorticoid target cells in the rat optic nerve. Hormone binding and glycerolphosphate dehydrogenase induction
    Meyer, Meyer; Leveille, Leveille; Vellis, Vellis; Gerlach, Gerlach; McEwen, McEwen
  • The attractions of proteins for small molecules and ions
    Scatchard, Scatchard

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off