Evaluation of microtitre plate‐based Haemoglobin estimation

Evaluation of microtitre plate‐based Haemoglobin estimation INTRODUCTIONAnaemia is a public health challenge in India, and it mostly affects women of reproductive age and preschool children. The District Level Health Survey (DLHS) 2002‐2004 reports an extremely high burden of anaemia with 98% of adolescent girls and around 96% of pregnant women suffering from this condition. Estimates of the prevalence of anaemia depend on the methods used for assessing haemoglobin concentration and on the cut‐off point applied as follows: the cut‐off point is different for different population screened for anaemia. Haemoglobin assessment is a reliable indicator for anaemia screening. However, there are multiple techniques with different applications available for haemoglobin estimation and each of the tests differs in cost, technical expertise required for the test and robustness of the value obtained.This study was carried out to develop a cyanmethaemoglobin‐based assay in microtitre plate for estimation of haemoglobin in field condition where large number of persons/population can be tested cosy effectively and accurately.MATERIALS AND METHODSStudy subjectsA total of 50 voluntary blood donors' EDTA samples were checked in house for haemoglobin concentration by automated blood cell counter and was used for standardization of microtitre plate assay. To check whether there is a difference between capillary blood haemoglobin concentration and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Laboratory Hematology Wiley

Evaluation of microtitre plate‐based Haemoglobin estimation

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 John Wiley & Sons Ltd
ISSN
1751-5521
eISSN
1751-553X
D.O.I.
10.1111/ijlh.12764
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONAnaemia is a public health challenge in India, and it mostly affects women of reproductive age and preschool children. The District Level Health Survey (DLHS) 2002‐2004 reports an extremely high burden of anaemia with 98% of adolescent girls and around 96% of pregnant women suffering from this condition. Estimates of the prevalence of anaemia depend on the methods used for assessing haemoglobin concentration and on the cut‐off point applied as follows: the cut‐off point is different for different population screened for anaemia. Haemoglobin assessment is a reliable indicator for anaemia screening. However, there are multiple techniques with different applications available for haemoglobin estimation and each of the tests differs in cost, technical expertise required for the test and robustness of the value obtained.This study was carried out to develop a cyanmethaemoglobin‐based assay in microtitre plate for estimation of haemoglobin in field condition where large number of persons/population can be tested cosy effectively and accurately.MATERIALS AND METHODSStudy subjectsA total of 50 voluntary blood donors' EDTA samples were checked in house for haemoglobin concentration by automated blood cell counter and was used for standardization of microtitre plate assay. To check whether there is a difference between capillary blood haemoglobin concentration and

Journal

International Journal of Laboratory HematologyWiley

Published: Jan 1, 2018

Keywords: ; ; ; ; ;

References

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