Evaluation of direct antifungal susceptibility testing methods of Candida spp. from positive blood culture bottles

Evaluation of direct antifungal susceptibility testing methods of Candida spp. from positive... INTRODUCTIONBloodstream infections (BSI) caused by Candida species are significant causes of morbidity and mortality, especially in intensive care unit patients and patients with hematological malignancy. Although Candida spp. were the fourth most common pathogens isolated from blood cultures, the crude mortality rate of Candida infections was the highest among all nosocomial BSI. Therefore, rapid and accurate diagnostic methods are essential for appropriate management of Candida BSIs. However, blood culture is still the gold standard diagnostic method in spite of long resulting time and low sensitivity rate in candidemia. The standard method of blood culture involves at least overnight agar medium subculture and then identification and antifungal susceptibility testing after a bottle is signaled as positive for Candida; it is time consuming and can delay the appropriate therapy. Several standard methods were suggested for antifungal susceptibility testing of yeast by both Clinical and Laboratory Standards Institute—CLSI (M27‐A3 and M44‐A2) and European Committee on Antimicrobial Susceptibility Testing—EUCAST (E.DEF 7.2). Besides, there are many commercial manual or automated systems such as Etest, Sensititre YeastOne, and Vitek which are used commonly in many clinical laboratories.Several studies including direct inoculation from positive blood culture bottles have been performed in order to decrease the time http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Laboratory Analysis Wiley

Evaluation of direct antifungal susceptibility testing methods of Candida spp. from positive blood culture bottles

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 Wiley Periodicals, Inc.
ISSN
0887-8013
eISSN
1098-2825
D.O.I.
10.1002/jcla.22297
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONBloodstream infections (BSI) caused by Candida species are significant causes of morbidity and mortality, especially in intensive care unit patients and patients with hematological malignancy. Although Candida spp. were the fourth most common pathogens isolated from blood cultures, the crude mortality rate of Candida infections was the highest among all nosocomial BSI. Therefore, rapid and accurate diagnostic methods are essential for appropriate management of Candida BSIs. However, blood culture is still the gold standard diagnostic method in spite of long resulting time and low sensitivity rate in candidemia. The standard method of blood culture involves at least overnight agar medium subculture and then identification and antifungal susceptibility testing after a bottle is signaled as positive for Candida; it is time consuming and can delay the appropriate therapy. Several standard methods were suggested for antifungal susceptibility testing of yeast by both Clinical and Laboratory Standards Institute—CLSI (M27‐A3 and M44‐A2) and European Committee on Antimicrobial Susceptibility Testing—EUCAST (E.DEF 7.2). Besides, there are many commercial manual or automated systems such as Etest, Sensititre YeastOne, and Vitek which are used commonly in many clinical laboratories.Several studies including direct inoculation from positive blood culture bottles have been performed in order to decrease the time

Journal

Journal of Clinical Laboratory AnalysisWiley

Published: Jan 1, 2018

Keywords: ; ; ; ; ;

References

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