Evaluation of a newly developed chemiluminescence immunoassay for detecting cardiac troponin T

Evaluation of a newly developed chemiluminescence immunoassay for detecting cardiac troponin T INTRODUCTIONTroponin (Tn) is an important regulatory protein necessary for striated muscle contraction. It consists of three subunits: troponin C (TnC), troponin I (TnI), and troponin T (TnT). TnT is the subunit that binds to tropomyosin and is classified into three subtypes: a fast skeletal muscle subtype, a slow skeletal muscle subtype, and a myocardial subtype. Cardiac troponin T (cTnT) is a 37 kD protein that is expressed with a high tissue specificity in myocardium and is approximately 10%‐30% structurally different from skeletal troponin. cTnT is present in the blood 3‐4 hours after a myocardial infarction, with levels remaining elevated for about 2 weeks. The unique molecular structure of cTnT, and the fact that it appears early and lasts longer in the blood upon cardiac muscle damage makes it a highly sensitive and specific marker of myocardial injury and cardiomyocyte death. Therefore, cTnT plays an important role in the early differential diagnosis and prognostic evaluation of acute myocardial infarction.At present, an electrochemiluminescence kit developed by Roche is prevalently used for cTnT clinical measurement in China. This kit is highly sensitive and specific for its utilized purpose, but the price of these imported reagents is high. Therefore, developing a domestically produced chemiluminescence immunoassay http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Clinical Laboratory Analysis Wiley

Evaluation of a newly developed chemiluminescence immunoassay for detecting cardiac troponin T

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Publisher
Wiley Subscription Services, Inc., A Wiley Company
Copyright
Copyright © 2018 Wiley Periodicals, Inc.
ISSN
0887-8013
eISSN
1098-2825
D.O.I.
10.1002/jcla.22311
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONTroponin (Tn) is an important regulatory protein necessary for striated muscle contraction. It consists of three subunits: troponin C (TnC), troponin I (TnI), and troponin T (TnT). TnT is the subunit that binds to tropomyosin and is classified into three subtypes: a fast skeletal muscle subtype, a slow skeletal muscle subtype, and a myocardial subtype. Cardiac troponin T (cTnT) is a 37 kD protein that is expressed with a high tissue specificity in myocardium and is approximately 10%‐30% structurally different from skeletal troponin. cTnT is present in the blood 3‐4 hours after a myocardial infarction, with levels remaining elevated for about 2 weeks. The unique molecular structure of cTnT, and the fact that it appears early and lasts longer in the blood upon cardiac muscle damage makes it a highly sensitive and specific marker of myocardial injury and cardiomyocyte death. Therefore, cTnT plays an important role in the early differential diagnosis and prognostic evaluation of acute myocardial infarction.At present, an electrochemiluminescence kit developed by Roche is prevalently used for cTnT clinical measurement in China. This kit is highly sensitive and specific for its utilized purpose, but the price of these imported reagents is high. Therefore, developing a domestically produced chemiluminescence immunoassay

Journal

Journal of Clinical Laboratory AnalysisWiley

Published: Jan 1, 2018

Keywords: ; ; ; ;

References

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