Ethanol production from cellulosic materials using cellulase‐expressing yeast

Ethanol production from cellulosic materials using cellulase‐expressing yeast We demonstrate direct ethanol fermentation from amorphous cellulose using cellulase‐co‐expressing yeast. Endoglucanases (EG) and cellobiohydrolases (CBH) from Trichoderma reesei, and β‐glucosidases (BGL) from Aspergillus aculeatus were integrated into genomes of the yeast strain Saccharomyces cerevisiae MT8‐1. BGL was displayed on the yeast cell surface and both EG and CBH were secreted or displayed on the cell surface. All enzymes were successfully expressed on the cell surface or in culture supernatants in their active forms, and cellulose degradation was increased 3‐ to 5‐fold by co‐expressing EG and CBH. Direct ethanol fermentation from 10 g/L phosphoric acid swollen cellulose (PASC) was also carried out using EG‐, CBH‐, and BGL‐co‐expressing yeast. The ethanol yield was 2.1 g/L for EG‐, CBH‐, and BGL‐displaying yeast, which was higher than that of EG‐ and CBH‐secreting yeast (1.6 g/L ethanol). Our results show that cell surface display is more suitable for direct ethanol fermentation from cellulose. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology Journal Wiley

Ethanol production from cellulosic materials using cellulase‐expressing yeast

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Publisher
Wiley
Copyright
Copyright © 2010 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1860-6768
eISSN
1860-7314
DOI
10.1002/biot.200900291
Publisher site
See Article on Publisher Site

Abstract

We demonstrate direct ethanol fermentation from amorphous cellulose using cellulase‐co‐expressing yeast. Endoglucanases (EG) and cellobiohydrolases (CBH) from Trichoderma reesei, and β‐glucosidases (BGL) from Aspergillus aculeatus were integrated into genomes of the yeast strain Saccharomyces cerevisiae MT8‐1. BGL was displayed on the yeast cell surface and both EG and CBH were secreted or displayed on the cell surface. All enzymes were successfully expressed on the cell surface or in culture supernatants in their active forms, and cellulose degradation was increased 3‐ to 5‐fold by co‐expressing EG and CBH. Direct ethanol fermentation from 10 g/L phosphoric acid swollen cellulose (PASC) was also carried out using EG‐, CBH‐, and BGL‐co‐expressing yeast. The ethanol yield was 2.1 g/L for EG‐, CBH‐, and BGL‐displaying yeast, which was higher than that of EG‐ and CBH‐secreting yeast (1.6 g/L ethanol). Our results show that cell surface display is more suitable for direct ethanol fermentation from cellulose.

Journal

Biotechnology JournalWiley

Published: May 1, 2010

Keywords: ; ; ; ;

References

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