ESTIMATION OF THE KINETIC PARAMETERS OF UNIDIRECTIONAL TRANSPORT ACROSS THE BLOOD‐BRAIN BARRIER

ESTIMATION OF THE KINETIC PARAMETERS OF UNIDIRECTIONAL TRANSPORT ACROSS THE BLOOD‐BRAIN BARRIER Abstract— The unidirectional transport of metabolic substrates from blood to brain may be defined in terms of Michaelis‐Menten saturable (Km, Vmax) and non‐saturable (Kd) components of influx. Various computation procedures have been previously reported to estimate the kinetic parameters when an intracarotid injection technique is used. Transformations of the influx data which allow linear plots to obtain estimates were compared with estimates obtained directly from a best fit on a least means squares criterion for both experimental and simulated data. Large discrepancies were apparent between the various estimates of the kinetic parameters when an equal weight was given to transformed data. For pyruvate (21‐day‐old rats), Km, values varied between 1.02 and 6.25 mM and Vmax varied between 0.68 and 2.30 μmol g−1 min−1. The estimates were almost equivalent when pyruvate data was re‐analysed using a weighting scheme based on the finding that the absolute value of the S.D. of influx increased in proportion to influx. It is recommended that estimates of kinetic parameters be obtained by an iterative, non‐linear least squares method to fit appropriately weighted data directly. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neurochemistry Wiley

ESTIMATION OF THE KINETIC PARAMETERS OF UNIDIRECTIONAL TRANSPORT ACROSS THE BLOOD‐BRAIN BARRIER

Journal of Neurochemistry, Volume 33 (2) – Aug 1, 1979

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Publisher
Wiley
Copyright
Copyright © 1979 Wiley Subscription Services, Inc., A Wiley Company
ISSN
0022-3042
eISSN
1471-4159
DOI
10.1111/j.1471-4159.1979.tb05172.x
Publisher site
See Article on Publisher Site

Abstract

Abstract— The unidirectional transport of metabolic substrates from blood to brain may be defined in terms of Michaelis‐Menten saturable (Km, Vmax) and non‐saturable (Kd) components of influx. Various computation procedures have been previously reported to estimate the kinetic parameters when an intracarotid injection technique is used. Transformations of the influx data which allow linear plots to obtain estimates were compared with estimates obtained directly from a best fit on a least means squares criterion for both experimental and simulated data. Large discrepancies were apparent between the various estimates of the kinetic parameters when an equal weight was given to transformed data. For pyruvate (21‐day‐old rats), Km, values varied between 1.02 and 6.25 mM and Vmax varied between 0.68 and 2.30 μmol g−1 min−1. The estimates were almost equivalent when pyruvate data was re‐analysed using a weighting scheme based on the finding that the absolute value of the S.D. of influx increased in proportion to influx. It is recommended that estimates of kinetic parameters be obtained by an iterative, non‐linear least squares method to fit appropriately weighted data directly.

Journal

Journal of NeurochemistryWiley

Published: Aug 1, 1979

References

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