Establishment and characterization of a human histiocytic lymphoma cell line (U‐937)

Establishment and characterization of a human histiocytic lymphoma cell line (U‐937) A human hematopoietic cell line (U‐937) with exceptional characteristics was derived from a patient with generalized histiocytic lymphoma. The morphology of the cell line was identical to that of the tumor cells in the pleural effusion from which the line was derived. Since Epstein‐Barr virus (EBV) carrying diploid lymphoblastoid cell lines unrelated to the tumor population often become established in vitro from non‐Burkitt lymphoma explants, several parameters were studied to discriminate the U‐937 from such lines: morphology in vitro, growth characteristics, cytochemistry, surface receptor pattern, Ig production, lysozyme production, β2‐micro‐globulin production, presence of EBV genome and karyotype. In all these respects U‐937 differed from prototype lymphoblastoid cell lines. The histiocytic origin of the cell line was shown by its capacity for lysozyme production and the strong esterase activity (naphtol AS‐D acetate esterase inhibited by NaF) of the cells. It is therefore concluded that the U‐937 is a neoplastic, histiocytic cell line. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Cancer Wiley

Establishment and characterization of a human histiocytic lymphoma cell line (U‐937)

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Publisher
Wiley
Copyright
Copyright © 1976 Wiley‐Liss, Inc., A Wiley Company
ISSN
0020-7136
eISSN
1097-0215
DOI
10.1002/ijc.2910170504
Publisher site
See Article on Publisher Site

Abstract

A human hematopoietic cell line (U‐937) with exceptional characteristics was derived from a patient with generalized histiocytic lymphoma. The morphology of the cell line was identical to that of the tumor cells in the pleural effusion from which the line was derived. Since Epstein‐Barr virus (EBV) carrying diploid lymphoblastoid cell lines unrelated to the tumor population often become established in vitro from non‐Burkitt lymphoma explants, several parameters were studied to discriminate the U‐937 from such lines: morphology in vitro, growth characteristics, cytochemistry, surface receptor pattern, Ig production, lysozyme production, β2‐micro‐globulin production, presence of EBV genome and karyotype. In all these respects U‐937 differed from prototype lymphoblastoid cell lines. The histiocytic origin of the cell line was shown by its capacity for lysozyme production and the strong esterase activity (naphtol AS‐D acetate esterase inhibited by NaF) of the cells. It is therefore concluded that the U‐937 is a neoplastic, histiocytic cell line.

Journal

International Journal of CancerWiley

Published: May 15, 1976

References

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