Equine laminitis: Membrane type matrix metalloproteinase‐1 (MMP‐14) is involved in acute phase onset

Equine laminitis: Membrane type matrix metalloproteinase‐1 (MMP‐14) is involved in acute... Summary Reasons for performing study: Enzymatic separation at the hoof lamellar dermal‐epidermal interface may play a role in the development of laminitis and characterising and locating matrix metalloproteinases (MMPs) and their inhibitors (tissue inhibitors of MMPs or TIMPs) in lamellar tissues may further understanding of pathogenesis. Objectives: To clone and sequence the cDNA encoding lamellar MMP‐14 and TIMP‐2, and quantify their transcription in normal and laminitic tissue; and to develop antibody to locate MMP‐14 in lamellar tissues. Methods: Tissue samples were obtained from an oligofructose induced model of laminitis. Total RNA was isolated, amplified by RT‐PCR, cloned into a vector and sequenced. Real‐time PCR was used to quantify MMP‐14 and TIMP‐2 expression. Rabbit anti‐equine MMP‐14 antibody was developed to analyse MMP‐14 proteins from hoof tissues. Results: Immunohistochemistry detected MMP‐14 in the cytoplasm of normal lamellar basal and parabasal cells in close proximity to the lamellar basement membrane. In laminitis affected tissue MMP‐14 immunostaining was depleted in lamellar basal cells. Quantitative real‐time PCR showed MMP‐14 and TIMP‐2 expression significantly (P<0.05) elevated and lowered respectively in laminitis affected tissues. Conclusion: MMP‐14, located in the cytoplasm of normal lamellar basal cells, disappears during laminitis development. The pathology of laminitis is associated with increased and lowered transcription of MMP‐14 and TIMP‐2, respectively. Potential relevance: Enzymes have a role in laminitis pathology and inhibition of their activity may prevent laminitis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Equine Veterinary Journal Wiley

Equine laminitis: Membrane type matrix metalloproteinase‐1 (MMP‐14) is involved in acute phase onset

Loading next page...
 
/lp/wiley/equine-laminitis-membrane-type-matrix-metalloproteinase-1-mmp-14-is-EWhknk3XJ6
Publisher
Wiley
Copyright
2008 EVJ Ltd
ISSN
0425-1644
eISSN
2042-3306
DOI
10.2746/042516408X270353
Publisher site
See Article on Publisher Site

Abstract

Summary Reasons for performing study: Enzymatic separation at the hoof lamellar dermal‐epidermal interface may play a role in the development of laminitis and characterising and locating matrix metalloproteinases (MMPs) and their inhibitors (tissue inhibitors of MMPs or TIMPs) in lamellar tissues may further understanding of pathogenesis. Objectives: To clone and sequence the cDNA encoding lamellar MMP‐14 and TIMP‐2, and quantify their transcription in normal and laminitic tissue; and to develop antibody to locate MMP‐14 in lamellar tissues. Methods: Tissue samples were obtained from an oligofructose induced model of laminitis. Total RNA was isolated, amplified by RT‐PCR, cloned into a vector and sequenced. Real‐time PCR was used to quantify MMP‐14 and TIMP‐2 expression. Rabbit anti‐equine MMP‐14 antibody was developed to analyse MMP‐14 proteins from hoof tissues. Results: Immunohistochemistry detected MMP‐14 in the cytoplasm of normal lamellar basal and parabasal cells in close proximity to the lamellar basement membrane. In laminitis affected tissue MMP‐14 immunostaining was depleted in lamellar basal cells. Quantitative real‐time PCR showed MMP‐14 and TIMP‐2 expression significantly (P<0.05) elevated and lowered respectively in laminitis affected tissues. Conclusion: MMP‐14, located in the cytoplasm of normal lamellar basal cells, disappears during laminitis development. The pathology of laminitis is associated with increased and lowered transcription of MMP‐14 and TIMP‐2, respectively. Potential relevance: Enzymes have a role in laminitis pathology and inhibition of their activity may prevent laminitis.

Journal

Equine Veterinary JournalWiley

Published: Jul 1, 2008

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off