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J. Dordick (1989)
Enzymatic catalysis in monophasic organic solventsEnzyme and Microbial Technology, 11
C. Laane, S. Boeren, K. Vos, C. Veeger (1987)
Rules for optimization of biocatalysis in organic solventsBiotechnology and Bioengineering, 30
I. Berezin, K. Martínek (1984)
Catalysis by Enzymes Entrapped in Reversed Micelles of Surfactants in Organic SolventsAnnals of the New York Academy of Sciences, 434
J. Carnali, A. Ceglie, B. Lindman, K. Shinoda (1986)
Structure of microemulsions in the brine/AOT/isooctane system at the HLB temperature studied by the self-diffusion techniqueLangmuir, 2
U. Olsson, K. Shinoda, B. Lindman (1986)
Change of the structure of microemulsions with the hydrophile-lipophile balance of nonionic surfactant as revealed by NMR self-diffusion studiesThe Journal of Physical Chemistry, 90
H. Dutler, C. Brändén (1981)
Correlation studies based on enzyme structure and kinetic results: Deduction of productive substrate orientation in the active-site pocket of horse liver alcohol dehydrogenaseBioorganic Chemistry, 10
K. Larsson, P. Adlercreutz, B. Mattiasson (1987)
Activity and stability of horse-liver alcohol dehydrogenase in sodium dioctylsulfosuccinate/cyclohexane reverse micelles.European journal of biochemistry, 166 1
H. Kunieda, K. Shinoda (1980)
Solution behavior and hydrophile-lipophile balance temperature in the aerosol OT-isooctane-brine system: Correlation between microemulsions and ultralow interfacial tensionsJournal of Colloid and Interface Science, 75
M. Kahlweit, R. Strey, P. Firman (1986)
Search for tricritical points in ternary systems: Water-oil-nonionic amphiphile.The Journal of Physical Chemistry, 90
K. Vos, C. Laane, A. Hoek, C. Veeger, A. Visser (1987)
Spectroscopic properties of horse liver alcohol dehydrogenase in reversed micellar solutions.European journal of biochemistry, 169 2
M. Dekker, K. Riet, S. Weijers, J.W.A. Baltussen, C. Laane, B. Bijsterbosch (1986)
Enzyme recovery by liquid-liquid extraction using reversed micelles.Chemical Engineering Journal, 33
P. Luisi, M. Giomini, M. Pileni, B. Robinson (1988)
Reverse micelles as hosts for proteins and small molecules.Biochimica et biophysica acta, 947 1
K. Shinoda, H. Kunieda (1987)
The effect of salt concentration, temperature, and additives on the solvent property of aerosol OT solution, 118
U. Olsson, K. Nagai, H. Wennerstroem (1988)
Microemulsions with nonionic surfactants. 1. Diffusion process of oil moleculesThe Journal of Physical Chemistry, 92
P. Luisi, P. Lüthi, I. Tomka, J. Přenosil, A. Pande (1984)
Hydrocarbon Micellar Solutions in Enzymatic Reactions of Apolar CompoundsAnnals of the New York Academy of Sciences, 434
J. Schulman, W. Stoeckenius, L. Prince (1959)
Mechanism of Formation and Structure of Micro Emulsions by Electron MicroscopyThe Journal of Physical Chemistry, 63
D. Eggers, H. Blanch (1988)
Enzymatic production of L-tryptophan in a reverse micelle reactorBioprocess Engineering, 3
P. Meier, P. Luisi (1980)
Micellar solubilization of biopolymers in hydrocarbon solvents. ii. the case of horse liver alcohol dehydrogenaseJournal of Solid-Phase Biochemistry, 5
M. Leser, G. Wei, P. Luisi, M. Maestro (1986)
Application of reverse micelles for the extraction of proteins.Biochemical and biophysical research communications, 135 2
L. Brink, J. Tramper, K. Luyben, K. Riet (1987)
Biocatalysis in Organic Media
Kangmin Lee, J. Biellmann (1987)
Enzyme and organic solvents: Horse liver alcohol dehydrogenase in non‐ionic microemulsion: Stability and activityFEBS Letters, 223
K. Martínek, I. Berezin, Y. Khmelnitski, N. Klyachko, A. Levashov (1987)
Enzymes Entrapped Into Reversed Micelles Of Surfactants In Organic Solvents: Key Trends In Applied Enzymology (Biotechnology), 1
J. Samama, K. Lee, J. Biellmann (1987)
Enzymes and microemulsions. Activity and kinetic properties of liver alcohol dehydrogenase in ionic water-in-oil microemulsions.European journal of biochemistry, 163 3
M. Kahlweit, R. Strey (1986)
Phase behavior of quinary systems water-oil-nonionic amphiphile-ionic amphiphile-electrolyteThe Journal of Physical Chemistry, 90
Volume 33
K. Larsson, C. Oldfield, R. Freedman (1989)
Analysis of the inactivation of liver alcohol dehydrogenase during storage in Aerosol-OT/isooctane microemulsions.European journal of biochemistry, 183 2
B. Lindman, K. Shinoda, U. Olsson, David Anderson, G. Karlström, H. Wennerström (1989)
On the demonstration of bicontinuous structures in microemulsionsColloids and Surfaces, 38
F. Lichterfeld, T. Schmeling, R. Strey (1986)
Microstructure of microemulsions of the system water-n-tetradecane-alkyl polyglycol ether (C12E5)The Journal of Physical Chemistry, 90
A. Zaks, A. Russell (1988)
Enzymes in organic solvents: properties and applicationsJournal of Biotechnology, 8
10.1002/bit.260360205.abs A technique for enzyme reuse and product recovery from enzymatic catalysis in microemulsions is demonstrated. The enzymatic reaction is performed in a homogeneous isotropic microemulsion; AOT (sodium bis‐(2‐ethyl‐ hexyl)sulfosuccinate)/isooctane/buffer or C12E5(penta ethylene glycol dodecyl ether)/heptane/buffer. By small temperature changes the systems are shifted to two phase regions, where an oil‐rich phase, containing the product, coexists with a water‐rich phase containing surfactant and enzyme. The oil‐rich phase may be replaced by an oil solution containing new substrate. Thus, the reaction may be continued and the enzyme reused. This procedure was repeated nine times in the present study. Data on phase behavior in presence and in absence of protein, partitioning of the components and a radioactive‐labelled protein between the phases, and the repeated use of horse liver alcohol dehydrogenase (HLADH) in the microemulsions are presented.
Biotechnology and Bioengineering – Wiley
Published: Jun 20, 1990
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