Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Enzymatic catalysis in microemulsions: Enzyme reuse and product recovery

Enzymatic catalysis in microemulsions: Enzyme reuse and product recovery 10.1002/bit.260360205.abs A technique for enzyme reuse and product recovery from enzymatic catalysis in microemulsions is demonstrated. The enzymatic reaction is performed in a homogeneous isotropic microemulsion; AOT (sodium bis‐(2‐ethyl‐ hexyl)sulfosuccinate)/isooctane/buffer or C12E5(penta ethylene glycol dodecyl ether)/heptane/buffer. By small temperature changes the systems are shifted to two phase regions, where an oil‐rich phase, containing the product, coexists with a water‐rich phase containing surfactant and enzyme. The oil‐rich phase may be replaced by an oil solution containing new substrate. Thus, the reaction may be continued and the enzyme reused. This procedure was repeated nine times in the present study. Data on phase behavior in presence and in absence of protein, partitioning of the components and a radioactive‐labelled protein between the phases, and the repeated use of horse liver alcohol dehydrogenase (HLADH) in the microemulsions are presented. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biotechnology and Bioengineering Wiley

Enzymatic catalysis in microemulsions: Enzyme reuse and product recovery

Loading next page...
 
/lp/wiley/enzymatic-catalysis-in-microemulsions-enzyme-reuse-and-product-tj625u0DWV

References (29)

Publisher
Wiley
Copyright
Copyright © 1990 John Wiley & Sons, Inc.
ISSN
0006-3592
eISSN
1097-0290
DOI
10.1002/bit.260360205
pmid
18595061
Publisher site
See Article on Publisher Site

Abstract

10.1002/bit.260360205.abs A technique for enzyme reuse and product recovery from enzymatic catalysis in microemulsions is demonstrated. The enzymatic reaction is performed in a homogeneous isotropic microemulsion; AOT (sodium bis‐(2‐ethyl‐ hexyl)sulfosuccinate)/isooctane/buffer or C12E5(penta ethylene glycol dodecyl ether)/heptane/buffer. By small temperature changes the systems are shifted to two phase regions, where an oil‐rich phase, containing the product, coexists with a water‐rich phase containing surfactant and enzyme. The oil‐rich phase may be replaced by an oil solution containing new substrate. Thus, the reaction may be continued and the enzyme reused. This procedure was repeated nine times in the present study. Data on phase behavior in presence and in absence of protein, partitioning of the components and a radioactive‐labelled protein between the phases, and the repeated use of horse liver alcohol dehydrogenase (HLADH) in the microemulsions are presented.

Journal

Biotechnology and BioengineeringWiley

Published: Jun 20, 1990

There are no references for this article.