Enhanced Mucosal Immune Responses Against Tetanus Toxoid Using Novel Delivery System Comprised of Chitosan‐Functionalized Gold Nanoparticles and Botanical Adjuvant: Characterization, Immunogenicity, and Stability Assessment

Enhanced Mucosal Immune Responses Against Tetanus Toxoid Using Novel Delivery System Comprised of... Approaches based on combined use of delivery systems and adjuvants are being favored to maximize efficient mucosal delivery of antigens. Here, we describe a novel delivery system comprised of chitosan‐functionalized gold nanoparticles (CsAuNPs) and saponin‐containing botanical adjuvant; Asparagus racemosus extract (ARE) for oral delivery of tetanus toxoid (TT). A significant increase in TT‐specific IgG (34.53‐fold) and IgA (43.75‐fold) was observed when TT–CsAuNPs were formulated with ARE (TT–ARE–CsAuNPs). The local IgA immune responses for TT also showed a significant increase (106.5‐fold in intestine washes and 99.74‐fold in feces) with ARE‐based formulations as compared with plain TT group. No effect of ARE was observed on size, charge, and loading properties of CsAuNPs. Additionally, no effect of ARE and CsAuNPs was observed on antigenicity and secondary structure of TT as determined by fluorescence, circular dichroism, and Fourier transform infrared spectroscopy. The stability studies demonstrated excellent stability profile of formulation at recommended storage conditions. The study establishes the possible role of immunomodulatory adjuvants in particulate delivery systems for mucosal delivery of vaccines. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:3448–3456, 2014 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Pharmaceutical Science Wiley

Enhanced Mucosal Immune Responses Against Tetanus Toxoid Using Novel Delivery System Comprised of Chitosan‐Functionalized Gold Nanoparticles and Botanical Adjuvant: Characterization, Immunogenicity, and Stability Assessment

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Publisher
Wiley
Copyright
Copyright © 2014 Wiley Periodicals, Inc., A Wiley Company
ISSN
0022-3549
eISSN
1520-6017
D.O.I.
10.1002/jps.24161
Publisher site
See Article on Publisher Site

Abstract

Approaches based on combined use of delivery systems and adjuvants are being favored to maximize efficient mucosal delivery of antigens. Here, we describe a novel delivery system comprised of chitosan‐functionalized gold nanoparticles (CsAuNPs) and saponin‐containing botanical adjuvant; Asparagus racemosus extract (ARE) for oral delivery of tetanus toxoid (TT). A significant increase in TT‐specific IgG (34.53‐fold) and IgA (43.75‐fold) was observed when TT–CsAuNPs were formulated with ARE (TT–ARE–CsAuNPs). The local IgA immune responses for TT also showed a significant increase (106.5‐fold in intestine washes and 99.74‐fold in feces) with ARE‐based formulations as compared with plain TT group. No effect of ARE was observed on size, charge, and loading properties of CsAuNPs. Additionally, no effect of ARE and CsAuNPs was observed on antigenicity and secondary structure of TT as determined by fluorescence, circular dichroism, and Fourier transform infrared spectroscopy. The stability studies demonstrated excellent stability profile of formulation at recommended storage conditions. The study establishes the possible role of immunomodulatory adjuvants in particulate delivery systems for mucosal delivery of vaccines. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:3448–3456, 2014

Journal

Journal of Pharmaceutical ScienceWiley

Published: Nov 1, 2014

References

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