Elimination of infectious retroviruses during preparation of immunoglobulins

Elimination of infectious retroviruses during preparation of immunoglobulins ABSTRACT: Safety concerns for immunoglobulin preparations have led us to study partition/inactivation of two prototype retroviruses, mouse xenotropic type C and lymphadenopathy‐associated virus (LAV) of the acquired immunodeficiency syndrome (AIDS), during manufacture and storage of immunoglobulins. Reduction of infectious retrovirus titers were 105 to 108‐fold through Cohn‐Oncley cold ethanol fractionation from plasma to fraction II, 103 to 105‐fold through incubation at pH 4.0 and another 104‐fold through incubation of the purified liquid immunoglobulin preparations at 27°C or 45°C. The results support the clinical and epidemiological evidence that therapeutic immunoglobulin preparations do not transmit AIDS virus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Transfusion Wiley

Elimination of infectious retroviruses during preparation of immunoglobulins

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Publisher
Wiley
Copyright
1986 AABB
ISSN
0041-1132
eISSN
1537-2995
D.O.I.
10.1046/j.1537-2995.1986.26486262753.x
Publisher site
See Article on Publisher Site

Abstract

ABSTRACT: Safety concerns for immunoglobulin preparations have led us to study partition/inactivation of two prototype retroviruses, mouse xenotropic type C and lymphadenopathy‐associated virus (LAV) of the acquired immunodeficiency syndrome (AIDS), during manufacture and storage of immunoglobulins. Reduction of infectious retrovirus titers were 105 to 108‐fold through Cohn‐Oncley cold ethanol fractionation from plasma to fraction II, 103 to 105‐fold through incubation at pH 4.0 and another 104‐fold through incubation of the purified liquid immunoglobulin preparations at 27°C or 45°C. The results support the clinical and epidemiological evidence that therapeutic immunoglobulin preparations do not transmit AIDS virus.

Journal

TransfusionWiley

Published: Jul 8, 1986

References

  • Human serum lysis RNA tumor viruses
    Welsh, Welsh; Cooper, Cooper; Jensen, Jensen

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