EGF enhances the survival of dopamine neurons in rat embryonic mesencephalon primary cell culture

EGF enhances the survival of dopamine neurons in rat embryonic mesencephalon primary cell culture Epidermal growth factor (EGF) immunoreactive material has been demonstrated to be present in the basal ganglia. In this study, we investigated the effect of EGF on cells cultured from 16‐day embryonic rat mesencephalon, which included dopamine neurons that project to the striatum in vivo. EGF receptors were detected in untreated cultures by (125I)‐EGF binding. Treatment of the cultures with EGF resulted in up to 50‐fold increases in neuronal high‐affinity dopamine uptake. Scatchard analysis of uptake kinetics and counting of tyrosine hydroxylase‐immunore‐active cells suggest that the effect of EGF on uptake is due to increased survival and maturation of dopaminergic neurons. By contrast, the high‐affinity uptake for serotonin was increased only threefold over its controls. There was no significant effect on high‐affinity γ‐aminobutyric acid (GABA) uptake. These results suggest that EGF is acting as a neurotrophic agent preferential for dopaminergic neurons in E16 mesencephalic cultures. Immunocytochemistry for glial flbrillary acidic protein demonstrated an increase in astroglia with EGF treatment. Fluorodeoxyuridine, an agent that is toxic to proliferating cells was able to eliminate the effect of EGF on dopamine uptake, suggesting that EGF may be increasing dopaminergic cell survival largely through a population of dividing cells. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Research Wiley

EGF enhances the survival of dopamine neurons in rat embryonic mesencephalon primary cell culture

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Publisher
Wiley
Copyright
Copyright © 1991 Wiley‐Liss, Inc.
ISSN
0360-4012
eISSN
1097-4547
D.O.I.
10.1002/jnr.490300213
Publisher site
See Article on Publisher Site

Abstract

Epidermal growth factor (EGF) immunoreactive material has been demonstrated to be present in the basal ganglia. In this study, we investigated the effect of EGF on cells cultured from 16‐day embryonic rat mesencephalon, which included dopamine neurons that project to the striatum in vivo. EGF receptors were detected in untreated cultures by (125I)‐EGF binding. Treatment of the cultures with EGF resulted in up to 50‐fold increases in neuronal high‐affinity dopamine uptake. Scatchard analysis of uptake kinetics and counting of tyrosine hydroxylase‐immunore‐active cells suggest that the effect of EGF on uptake is due to increased survival and maturation of dopaminergic neurons. By contrast, the high‐affinity uptake for serotonin was increased only threefold over its controls. There was no significant effect on high‐affinity γ‐aminobutyric acid (GABA) uptake. These results suggest that EGF is acting as a neurotrophic agent preferential for dopaminergic neurons in E16 mesencephalic cultures. Immunocytochemistry for glial flbrillary acidic protein demonstrated an increase in astroglia with EGF treatment. Fluorodeoxyuridine, an agent that is toxic to proliferating cells was able to eliminate the effect of EGF on dopamine uptake, suggesting that EGF may be increasing dopaminergic cell survival largely through a population of dividing cells.

Journal

Journal of Neuroscience ResearchWiley

Published: Oct 1, 1991

References

  • Effect of epidermal growth factor and insulin on DNA, RNA, and cytoskeletal protein labeling in primary rat astroglial cell cultures
    Avola, Avola; Condorelli, Condorelli; Surrentino, Surrentino; Turpeenoja, Turpeenoja; Costa, Costa; Giuffrida Stella, Giuffrida Stella
  • Tyrosine hydroxylase in rat brain: Developmental characteristics
    Coyle, Coyle; Axelrod, Axelrod
  • Epidermal growth factor in synaptosomal fraction of mouse cerebral cortex
    Lakshmanan, Lakshmanan; Weichsel, Weichsel; Fisher, Fisher
  • Epidermal growth factor stimulates DNA‐synthesis of astrocytes in primary cerebellar cultures
    Leutz, Leutz; Schachner, Schachner
  • Basic fibroblast growth factor and epidermal growth factor exert differential trophic effects on CNS neurons
    Morrison, Morrison; Keating, Keating; Moskal, Moskal
  • A soluble brain molecule related to epidermal growth factor receptor is a mitogen inhibitor for astrocytes
    Nieto‐Sampedro, Nieto‐Sampedro; Broderick, Broderick
  • The attractions of protein for small molecules and ions
    Scatchard, Scatchard
  • Epidermal growth factor binding and mitogenic activity on purified populations of cells from the central nervous system
    Simpson, Simpson; Morrison, Morrison; De Vellis, De Vellis; Herschman, Herschman
  • The selective neuronal uptake and release of ( 3 H) DL‐2,4‐diaminobutyric acid by rat cerebral cortex
    Weitch Dick, Weitch Dick; Jessel, Jessel; Kelly, Kelly

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